Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11202 | 3' | -50.5 | NC_002816.1 | + | 93261 | 0.67 | 0.974111 |
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Target: 5'- aCGCCACAAUCAccuugguuggGUUGCGCaCAUc-- -3' miRNA: 3'- -GCGGUGUUAGUa---------CAGCGCGaGUAaca -5' |
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| 11202 | 3' | -50.5 | NC_002816.1 | + | 120838 | 0.68 | 0.957292 |
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Target: 5'- uCGCuCACcaauuuuuucaAGUCAUcaaCGCGCUCAUUGUa -3' miRNA: 3'- -GCG-GUG-----------UUAGUAca-GCGCGAGUAACA- -5' |
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| 11202 | 3' | -50.5 | NC_002816.1 | + | 68235 | 0.7 | 0.917181 |
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Target: 5'- -aUCACGAUCcagGUCGCGCUCGagGUc -3' miRNA: 3'- gcGGUGUUAGua-CAGCGCGAGUaaCA- -5' |
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| 11202 | 3' | -50.5 | NC_002816.1 | + | 44806 | 0.71 | 0.890749 |
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Target: 5'- aGCCACAuUCAuuUGUUugGCGCUC-UUGUa -3' miRNA: 3'- gCGGUGUuAGU--ACAG--CGCGAGuAACA- -5' |
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| 11202 | 3' | -50.5 | NC_002816.1 | + | 12016 | 0.73 | 0.807758 |
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Target: 5'- aGCCACcagAGUCGUG-CGCGUUCAUuuggUGUa -3' miRNA: 3'- gCGGUG---UUAGUACaGCGCGAGUA----ACA- -5' |
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| 11202 | 3' | -50.5 | NC_002816.1 | + | 93033 | 1.09 | 0.006844 |
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Target: 5'- aCGCCACAAUCAUGUCGCGCUCAUUGUg -3' miRNA: 3'- -GCGGUGUUAGUACAGCGCGAGUAACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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