miRNA: 3'- -UGUGUUau---UAGAGUGGcaGGUGC-GCG- -5'
miRNA: 3'- -UGUGUUaUUAGa-GUGGCAGGUGcGCG- -5'
miRNA: 3'- uGUGUUAuua-GAGug------GCAGGUGCGCG- -5'
miRNA: 3'- -UGUGUu---AUUAGa----GUGGCAGG---UGC-GCG- -5'
miRNA: 3'- -UGUGUUAUU---AGAGUGGCag---GUGCGcg -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11202 | 5' | -49.7 | NC_002816.1 | + | 101289 | 0.67 | 0.989841 |
|
Target: 5'- aGCGCGAccgauGUCUCGCCa-UCACGcCGCg -3' miRNA: 3'- -UGUGUUau---UAGAGUGGcaGGUGC-GCG- -5' |
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| 11202 | 5' | -49.7 | NC_002816.1 | + | 59137 | 0.67 | 0.992303 |
|
Target: 5'- aACGCGA-AAUCgcCACCGUUUACcCGCa -3' miRNA: 3'- -UGUGUUaUUAGa-GUGGCAGGUGcGCG- -5' |
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| 11202 | 5' | -49.7 | NC_002816.1 | + | 107690 | 0.66 | 0.993342 |
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Target: 5'- uCACGAUucagCUUggugggugCGUCCACGCGCu -3' miRNA: 3'- uGUGUUAuua-GAGug------GCAGGUGCGCG- -5' |
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| 11202 | 5' | -49.7 | NC_002816.1 | + | 45709 | 0.66 | 0.995803 |
|
Target: 5'- gACACAuacuUGAUCaccugCGCCGUCUugaACGcCGUg -3' miRNA: 3'- -UGUGUu---AUUAGa----GUGGCAGG---UGC-GCG- -5' |
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| 11202 | 5' | -49.7 | NC_002816.1 | + | 13581 | 0.74 | 0.806488 |
|
Target: 5'- gACACGGUAGuguUCUCACCGgguaaCACGCa- -3' miRNA: 3'- -UGUGUUAUU---AGAGUGGCag---GUGCGcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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