Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11202 | 5' | -49.7 | NC_002816.1 | + | 68444 | 0.66 | 0.994 |
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Target: 5'- uACACAAU-AUCUgACaaaugacacugcuuCGUcgcCCGCGCGCa -3' miRNA: 3'- -UGUGUUAuUAGAgUG--------------GCA---GGUGCGCG- -5' |
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| 11202 | 5' | -49.7 | NC_002816.1 | + | 83103 | 0.66 | 0.994928 |
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Target: 5'- uCACucaccgcCUCACCGUCCAuaaacuuuuuCGCGUc -3' miRNA: 3'- uGUGuuauua-GAGUGGCAGGU----------GCGCG- -5' |
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| 11202 | 5' | -49.7 | NC_002816.1 | + | 6281 | 0.66 | 0.995083 |
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Target: 5'- uACACAcgu-UC-CGCCGUCUuucuguACGCGUa -3' miRNA: 3'- -UGUGUuauuAGaGUGGCAGG------UGCGCG- -5' |
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| 11202 | 5' | -49.7 | NC_002816.1 | + | 45709 | 0.66 | 0.995803 |
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Target: 5'- gACACAuacuUGAUCaccugCGCCGUCUugaACGcCGUg -3' miRNA: 3'- -UGUGUu---AUUAGa----GUGGCAGG---UGC-GCG- -5' |
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| 11202 | 5' | -49.7 | NC_002816.1 | + | 27199 | 0.66 | 0.995803 |
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Target: 5'- aACACGuuauGUGgcGUgUUugUGUCCGCGuCGCg -3' miRNA: 3'- -UGUGU----UAU--UAgAGugGCAGGUGC-GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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