Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11265 | 5' | -55.9 | NC_003059.1 | + | 622 | 0.66 | 0.188043 |
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Target: 5'- gUCCAUucGGGAagcgguguGGC-CAAGGCCa -3' miRNA: 3'- gGGGUAucCCCUguu-----CCGuGUUCCGG- -5' |
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| 11265 | 5' | -55.9 | NC_003059.1 | + | 2611 | 0.66 | 0.178935 |
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Target: 5'- gCCUAgcggagucugacugGGGGGACcAGGCucucuccaACuGGGCCg -3' miRNA: 3'- gGGGUa-------------UCCCCUGuUCCG--------UGuUCCGG- -5' |
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| 11265 | 5' | -55.9 | NC_003059.1 | + | 512 | 0.67 | 0.166008 |
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Target: 5'- cCCCCAguucccaGACAAcgguGGCACAagAGGCCa -3' miRNA: 3'- -GGGGUauccc--CUGUU----CCGUGU--UCCGG- -5' |
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| 11265 | 5' | -55.9 | NC_003059.1 | + | 2913 | 0.67 | 0.155882 |
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Target: 5'- uUCCCAUcAGGGGAacuGGGCAguCAguuAGGUa -3' miRNA: 3'- -GGGGUA-UCCCCUgu-UCCGU--GU---UCCGg -5' |
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| 11265 | 5' | -55.9 | NC_003059.1 | + | 5070 | 0.68 | 0.139055 |
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Target: 5'- uCCUCuggagaauauuuGGGGACAGcuGGCAUuucuGGGGCCa -3' miRNA: 3'- -GGGGuau---------CCCCUGUU--CCGUG----UUCCGG- -5' |
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| 11265 | 5' | -55.9 | NC_003059.1 | + | 5963 | 0.68 | 0.126324 |
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Target: 5'- cCCCCAcaagcUGGGGGACcaggugugauaAAGGaCugGaaaaaugagcccuugAGGCCa -3' miRNA: 3'- -GGGGU-----AUCCCCUG-----------UUCC-GugU---------------UCCGG- -5' |
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| 11265 | 5' | -55.9 | NC_003059.1 | + | 2454 | 0.7 | 0.084102 |
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Target: 5'- uCCCCuauGGGGugGucccacuggGGGCACAaguuuugacagggAGGCUg -3' miRNA: 3'- -GGGGuauCCCCugU---------UCCGUGU-------------UCCGG- -5' |
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| 11265 | 5' | -55.9 | NC_003059.1 | + | 2435 | 1.15 | 1.7e-05 |
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Target: 5'- aCCCCAUAGGGGACAAGGCACAAGGCCa -3' miRNA: 3'- -GGGGUAUCCCCUGUUCCGUGUUCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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