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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11268 | 3' | -49.9 | NC_003059.1 | + | 2442 | 0.66 | 0.437913 |
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Target: 5'- aGGGGACAAggcacaaGGCCAGGgAAAaggaguccCCUGGa -3' miRNA: 3'- -CCCCUGUUg------UCGGUCUgUUU--------GGAUUc -5' |
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| 11268 | 3' | -49.9 | NC_003059.1 | + | 6452 | 0.66 | 0.436747 |
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Target: 5'- gGGGGAacaAACacaggagaccacuGGCCuuGGCAAGCCaGAGa -3' miRNA: 3'- -CCCCUg--UUG-------------UCGGu-CUGUUUGGaUUC- -5' |
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| 11268 | 3' | -49.9 | NC_003059.1 | + | 6599 | 0.66 | 0.435583 |
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Target: 5'- uGGGGAgGACAGUUuaauguuugaguuGGAagccuaacauguuUAGGCCUAAGg -3' miRNA: 3'- -CCCCUgUUGUCGG-------------UCU-------------GUUUGGAUUC- -5' |
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| 11268 | 3' | -49.9 | NC_003059.1 | + | 5054 | 0.74 | 0.131807 |
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Target: 5'- uGGGGACAGCuggcauuucuggGGCCAGcaAGACCUuAGg -3' miRNA: 3'- -CCCCUGUUG------------UCGGUCugUUUGGAuUC- -5' |
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| 11268 | 3' | -49.9 | NC_003059.1 | + | 4987 | 1.08 | 0.000278 |
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Target: 5'- cGGGACAACAGCCAGACAAACCUAAGg -3' miRNA: 3'- cCCCUGUUGUCGGUCUGUUUGGAUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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