miRNA: 3'- aaacGCAACUGGUUauauCGCGGCCG- -5'
miRNA: 3'- aaaCGCA---AC---UGGUUAUAUCgCGGcCG- -5'
miRNA: 3'- aaaCGCaa-CUGGuuauaUCGCGGCCG- -5'
miRNA: 3'- aaaCGCaACUGgUuaUAUCGCGGCCG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11282 | 3' | -50.4 | NC_003083.1 | + | 89753 | 0.67 | 0.971917 |
|
Target: 5'- ---uUGUUGACUAAcaccGCGCUGGCg -3' miRNA: 3'- aaacGCAACUGGUUauauCGCGGCCG- -5' |
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| 11282 | 3' | -50.4 | NC_003083.1 | + | 93211 | 0.66 | 0.986052 |
|
Target: 5'- --cGCGUcuaUGgcuACCGGUAUGGgGCCuGCu -3' miRNA: 3'- aaaCGCA---AC---UGGUUAUAUCgCGGcCG- -5' |
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| 11282 | 3' | -50.4 | NC_003083.1 | + | 114174 | 0.69 | 0.940066 |
|
Target: 5'- --aGUGgacGACCugcggGGUGCCGGCa -3' miRNA: 3'- aaaCGCaa-CUGGuuauaUCGCGGCCG- -5' |
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| 11282 | 3' | -50.4 | NC_003083.1 | + | 118307 | 0.68 | 0.958037 |
|
Target: 5'- --gGCGgUGGCgGggGUGGUGCUGGUa -3' miRNA: 3'- aaaCGCaACUGgUuaUAUCGCGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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