Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11288 | 3' | -57 | NC_003083.1 | + | 36185 | 0.66 | 0.841638 |
Target: 5'- cGACGcGCA-GUGcCACCCGUaaACGUCa -3' miRNA: 3'- uCUGC-CGUgCAU-GUGGGCAgcUGCGG- -5' |
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11288 | 3' | -57 | NC_003083.1 | + | 24144 | 0.66 | 0.864965 |
Target: 5'- cGACGGCGUGUgucAUACUCGcCGcccaacACGCCg -3' miRNA: 3'- uCUGCCGUGCA---UGUGGGCaGC------UGCGG- -5' |
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11288 | 3' | -57 | NC_003083.1 | + | 24052 | 0.67 | 0.807942 |
Target: 5'- cGACGGUGCGUugGCaa-UCGGCuugGCCa -3' miRNA: 3'- uCUGCCGUGCAugUGggcAGCUG---CGG- -5' |
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11288 | 3' | -57 | NC_003083.1 | + | 23999 | 0.75 | 0.354501 |
Target: 5'- uGGGCGGCGaGUaugacACACgCCGUCGACGUa -3' miRNA: 3'- -UCUGCCGUgCA-----UGUG-GGCAGCUGCGg -5' |
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11288 | 3' | -57 | NC_003083.1 | + | 8801 | 0.66 | 0.864217 |
Target: 5'- uGGCGGCugGUgauuuaagagaccGCGCCgGguaagUGAUGUCa -3' miRNA: 3'- uCUGCCGugCA-------------UGUGGgCa----GCUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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