miRNA: 3'- auaGUGaua--CAUuCGuGCUCACCUGG- -5'
miRNA: 3'- auaGUGAUacauUCGUGCU-------CACCUGG- -5'
miRNA: 3'- auagugaUACAUUCGU----------GCUCACCUGG- -5'
miRNA: 3'- aUAGUGAUACAU----UCGUGcUCaCCUGG- -5'
miRNA: 3'- auagUGAUa--CAUUCGUgCUCACCUGG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11315 | 3' | -47.9 | NC_003084.1 | + | 65469 | 0.66 | 0.99738 |
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Target: 5'- --cCACcguugGUGuGC-CGAGUGGGCCu -3' miRNA: 3'- auaGUGaua--CAUuCGuGCUCACCUGG- -5' |
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| 11315 | 3' | -47.9 | NC_003084.1 | + | 92578 | 0.66 | 0.997777 |
|
Target: 5'- --cCGCUAcuu-AGCACGAcuuuaccGUGGACCc -3' miRNA: 3'- auaGUGAUacauUCGUGCU-------CACCUGG- -5' |
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| 11315 | 3' | -47.9 | NC_003084.1 | + | 68975 | 0.66 | 0.997818 |
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Target: 5'- ------aAUGUGAGCAccacucauguCGGGUGGACUu -3' miRNA: 3'- auagugaUACAUUCGU----------GCUCACCUGG- -5' |
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| 11315 | 3' | -47.9 | NC_003084.1 | + | 60635 | 0.66 | 0.998193 |
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Target: 5'- aGUCGCUGUGUAgggcGGCAC-AGcGGGUCa -3' miRNA: 3'- aUAGUGAUACAU----UCGUGcUCaCCUGG- -5' |
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| 11315 | 3' | -47.9 | NC_003084.1 | + | 62776 | 0.66 | 0.998193 |
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Target: 5'- ---aGCUGaagGUGAGUG-GGGUGGACCc -3' miRNA: 3'- auagUGAUa--CAUUCGUgCUCACCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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