miRNA: 3'- auAGUGauaCaUUCGuGCUCACCUGG- -5'
miRNA: 3'- auaGUGAUacauUCGUGCU-------CACCUGG- -5'
miRNA: 3'- aUAGugAuaCAU-------UCGUGCUCACCUgG- -5'
miRNA: 3'- auaGUGAUACAUUCgu---GCUCACCUGg -5'
miRNA: 3'- auaGUGAUaCAUUCGuGCUCA---CCUGG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11315 | 3' | -47.9 | NC_003084.1 | + | 82485 | 0.68 | 0.987601 |
|
Target: 5'- -cUCAUcg-GcAAGC-CGAGUGGACCc -3' miRNA: 3'- auAGUGauaCaUUCGuGCUCACCUGG- -5' |
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| 11315 | 3' | -47.9 | NC_003084.1 | + | 92578 | 0.66 | 0.997777 |
|
Target: 5'- --cCGCUAcuu-AGCACGAcuuuaccGUGGACCc -3' miRNA: 3'- auaGUGAUacauUCGUGCU-------CACCUGG- -5' |
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| 11315 | 3' | -47.9 | NC_003084.1 | + | 94431 | 0.72 | 0.926782 |
|
Target: 5'- cAUCugUggGUAaacugguGGUACGAGUGGAgCg -3' miRNA: 3'- aUAGugAuaCAU-------UCGUGCUCACCUgG- -5' |
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| 11315 | 3' | -47.9 | NC_003084.1 | + | 106427 | 0.71 | 0.943409 |
|
Target: 5'- --cCACUGUGUGGGaccugUGGGUGGACa -3' miRNA: 3'- auaGUGAUACAUUCgu---GCUCACCUGg -5' |
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| 11315 | 3' | -47.9 | NC_003084.1 | + | 106891 | 0.73 | 0.872236 |
|
Target: 5'- --gCACUGaGUGAGCcCGAGUugaGGGCCa -3' miRNA: 3'- auaGUGAUaCAUUCGuGCUCA---CCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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