Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11315 | 5' | -55.9 | NC_003084.1 | + | 50929 | 0.68 | 0.708497 |
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Target: 5'- gAGGACUgcauacauuCCCGCuACcACAUCGAGUUGg -3' miRNA: 3'- -UCCUGGu--------GGGCG-UGcUGUAGUUCGAC- -5' |
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| 11315 | 5' | -55.9 | NC_003084.1 | + | 107568 | 0.69 | 0.677395 |
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Target: 5'- aAGGACCACCCgaagguucgcucGCACGAgggaCGUCuaguGCUa -3' miRNA: 3'- -UCCUGGUGGG------------CGUGCU----GUAGuu--CGAc -5' |
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| 11315 | 5' | -55.9 | NC_003084.1 | + | 1666 | 0.69 | 0.64594 |
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Target: 5'- uGGGACCgaagcACCCGCACcuggccucCAUgGAGCUGc -3' miRNA: 3'- -UCCUGG-----UGGGCGUGcu------GUAgUUCGAC- -5' |
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| 11315 | 5' | -55.9 | NC_003084.1 | + | 105266 | 0.72 | 0.521263 |
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Target: 5'- cGGugUACgCCGCACGAC--CGAGUUGg -3' miRNA: 3'- uCCugGUG-GGCGUGCUGuaGUUCGAC- -5' |
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| 11315 | 5' | -55.9 | NC_003084.1 | + | 42690 | 0.72 | 0.491365 |
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Target: 5'- gGGGugCGCCUG-ACgGACAUCGAGCg- -3' miRNA: 3'- -UCCugGUGGGCgUG-CUGUAGUUCGac -5' |
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| 11315 | 5' | -55.9 | NC_003084.1 | + | 22220 | 0.73 | 0.434138 |
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Target: 5'- uGGugCGCCUGCugGACucgguuAUCGAGCg- -3' miRNA: 3'- uCCugGUGGGCGugCUG------UAGUUCGac -5' |
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| 11315 | 5' | -55.9 | NC_003084.1 | + | 7966 | 1.08 | 0.002342 |
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Target: 5'- gAGGACCACCCGCACGACAUCAAGCUGc -3' miRNA: 3'- -UCCUGGUGGGCGUGCUGUAGUUCGAC- -5' |
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| 11315 | 5' | -55.9 | NC_003084.1 | + | 102200 | 0.66 | 0.844492 |
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Target: 5'- gGGGGCCACCCGguaCAgGugGUCGuacgugcccgccauuAGCa- -3' miRNA: 3'- -UCCUGGUGGGC---GUgCugUAGU---------------UCGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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