Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11319 | 5' | -49.9 | NC_003084.1 | + | 67205 | 0.66 | 0.995144 |
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Target: 5'- gUCCACCACCGU--GAUCUGcgcgUCGg -3' miRNA: 3'- -GGGUGGUGGCAaaUUGGGCuaaaGGC- -5' |
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| 11319 | 5' | -49.9 | NC_003084.1 | + | 68225 | 0.69 | 0.96459 |
|
Target: 5'- gUCCACCAgCGUauccaagcugUAGCCCGGUggaaccaacguUUCCa -3' miRNA: 3'- -GGGUGGUgGCAa---------AUUGGGCUA-----------AAGGc -5' |
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| 11319 | 5' | -49.9 | NC_003084.1 | + | 69078 | 0.67 | 0.985994 |
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Target: 5'- uUCCACCACaCauuuugcgcuacaagGUgUGACCCGGggUCCa -3' miRNA: 3'- -GGGUGGUG-G---------------CAaAUUGGGCUaaAGGc -5' |
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| 11319 | 5' | -49.9 | NC_003084.1 | + | 82501 | 0.66 | 0.995068 |
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Target: 5'- uUCCACCucuGCCGUguucacgUUAcuACCCGGcgUCCu -3' miRNA: 3'- -GGGUGG---UGGCA-------AAU--UGGGCUaaAGGc -5' |
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| 11319 | 5' | -49.9 | NC_003084.1 | + | 85185 | 0.69 | 0.961021 |
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Target: 5'- cCCCaACCGCgGU--GGuuCGAUUUCCGa -3' miRNA: 3'- -GGG-UGGUGgCAaaUUggGCUAAAGGC- -5' |
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| 11319 | 5' | -49.9 | NC_003084.1 | + | 90262 | 0.71 | 0.915131 |
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Target: 5'- gCCCACCAgCGguguaacggugGACCCGAaugcgUUUCCc -3' miRNA: 3'- -GGGUGGUgGCaaa--------UUGGGCU-----AAAGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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