Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11414 | 5' | -51.1 | NC_003085.1 | + | 48810 | 0.73 | 0.516158 |
Target: 5'- aCGUCCgGGCGguGCUCGAGcGccUCGACg -3' miRNA: 3'- cGUAGGgUUGCguUGAGCUC-C--AGUUG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 45783 | 1.12 | 0.001494 |
Target: 5'- cGCAUCCCAACGCAACUCGAGGUCAACc -3' miRNA: 3'- -CGUAGGGUUGCGUUGAGCUCCAGUUG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 43574 | 0.67 | 0.850856 |
Target: 5'- cGCGUcaccgaCCCGGCGCcACggcaGAGGUCGcagGCg -3' miRNA: 3'- -CGUA------GGGUUGCGuUGag--CUCCAGU---UG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 43404 | 0.74 | 0.452547 |
Target: 5'- uGCAUCCCggUGgGGCUCGggagaaucAGGUCAu- -3' miRNA: 3'- -CGUAGGGuuGCgUUGAGC--------UCCAGUug -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 43202 | 0.68 | 0.773254 |
Target: 5'- cGCAUCCCAcgGCGGCgUCGuguaGGcGUCGACc -3' miRNA: 3'- -CGUAGGGUugCGUUG-AGC----UC-CAGUUG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 37730 | 0.7 | 0.708132 |
Target: 5'- uGCccCUCGACaGCAccACgUCGAGGUCGGCg -3' miRNA: 3'- -CGuaGGGUUG-CGU--UG-AGCUCCAGUUG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 36811 | 0.66 | 0.867991 |
Target: 5'- uCGUCCCGcACGCucuucagCGAGG-CAGCg -3' miRNA: 3'- cGUAGGGU-UGCGuuga---GCUCCaGUUG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 33205 | 0.7 | 0.662935 |
Target: 5'- gGCGUCCUggaggcggcGGCGCAGCaCGAGGaugaCGACg -3' miRNA: 3'- -CGUAGGG---------UUGCGUUGaGCUCCa---GUUG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 32453 | 0.67 | 0.859552 |
Target: 5'- aGCAgcgCCCAuccGCGCuuCUCGucgccGUCGGCg -3' miRNA: 3'- -CGUa--GGGU---UGCGuuGAGCuc---CAGUUG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 32406 | 0.66 | 0.899033 |
Target: 5'- cGUAcuUCUCGACGCccucgaAGCUCcgGAGGUUGGCg -3' miRNA: 3'- -CGU--AGGGUUGCG------UUGAG--CUCCAGUUG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 30837 | 0.71 | 0.640102 |
Target: 5'- gGCAaCCCAGCaGCAACUCGA---CAACg -3' miRNA: 3'- -CGUaGGGUUG-CGUUGAGCUccaGUUG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 26697 | 0.66 | 0.891693 |
Target: 5'- cCGUCgCCAcCGCGGCgggCGucGUCGGCg -3' miRNA: 3'- cGUAG-GGUuGCGUUGa--GCucCAGUUG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 23458 | 0.79 | 0.229178 |
Target: 5'- cGCAUgacggaggCCCAGCGCAGCgUCGAGGcauucgUCAGCa -3' miRNA: 3'- -CGUA--------GGGUUGCGUUG-AGCUCC------AGUUG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 21155 | 0.66 | 0.867991 |
Target: 5'- cCGUCCCAccGCGCAccuGCUgCGAGGcCugguACu -3' miRNA: 3'- cGUAGGGU--UGCGU---UGA-GCUCCaGu---UG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 20668 | 0.67 | 0.850856 |
Target: 5'- gGCAcUCCUcgcgcCGCAACUCGugcacaacgAGGUCAAUu -3' miRNA: 3'- -CGU-AGGGuu---GCGUUGAGC---------UCCAGUUG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 19546 | 0.67 | 0.839183 |
Target: 5'- cGCG-CCCGACGCGGagaaccuggagaacCUCccGGUCGGCu -3' miRNA: 3'- -CGUaGGGUUGCGUU--------------GAGcuCCAGUUG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 18336 | 0.71 | 0.617238 |
Target: 5'- cGCAcgaaCUCGGCGCGcuGCUCGGGGUUGAg -3' miRNA: 3'- -CGUa---GGGUUGCGU--UGAGCUCCAGUUg -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 17931 | 0.67 | 0.850856 |
Target: 5'- uGCG-CCCAGCGCGgcGCUCuGGGcUCuGCu -3' miRNA: 3'- -CGUaGGGUUGCGU--UGAGcUCC-AGuUG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 16443 | 0.66 | 0.867991 |
Target: 5'- gGCG--CCGACGUcAUUCGAGG-CGACg -3' miRNA: 3'- -CGUagGGUUGCGuUGAGCUCCaGUUG- -5' |
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11414 | 5' | -51.1 | NC_003085.1 | + | 15568 | 0.66 | 0.867991 |
Target: 5'- cGCGUCaCUggUGCGAUUUcagGAGGUCGc- -3' miRNA: 3'- -CGUAG-GGuuGCGUUGAG---CUCCAGUug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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