Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11418 | 3' | -58.3 | NC_003085.1 | + | 46354 | 0.66 | 0.534899 |
Target: 5'- -gAGAUGGCgGcaAGUucaUCCGCCGUCGGc -3' miRNA: 3'- caUCUGCCGgC--UCA---GGGCGGUAGCUc -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 21934 | 0.66 | 0.534898 |
Target: 5'- -gAGAUGGCCacgaggGAGUCaucaugccggcgCCGCCGUCGu- -3' miRNA: 3'- caUCUGCCGG------CUCAG------------GGCGGUAGCuc -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 32396 | 0.66 | 0.514088 |
Target: 5'- uGUGGGCGGCCGuacuUCUcgaCGCCcUCGAa -3' miRNA: 3'- -CAUCUGCCGGCuc--AGG---GCGGuAGCUc -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 40843 | 0.66 | 0.503806 |
Target: 5'- --cGGCGGCCGGG-CUgGCCAaccUgGAGg -3' miRNA: 3'- cauCUGCCGGCUCaGGgCGGU---AgCUC- -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 31335 | 0.67 | 0.493615 |
Target: 5'- -cAGACgucccaGGCCGGccGUCCCGCCcgCGc- -3' miRNA: 3'- caUCUG------CCGGCU--CAGGGCGGuaGCuc -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 810 | 0.67 | 0.482515 |
Target: 5'- gGUGGACGuCCGcGUgCCGCCgcaggucGUCGAGc -3' miRNA: 3'- -CAUCUGCcGGCuCAgGGCGG-------UAGCUC- -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 24086 | 0.67 | 0.463632 |
Target: 5'- --cGACGGUgaAG-CCCGCCGUCGGc -3' miRNA: 3'- cauCUGCCGgcUCaGGGCGGUAGCUc -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 17728 | 0.67 | 0.463632 |
Target: 5'- -gAGAgaGGCgCGugaccAGUCCCGCCuUCGGGa -3' miRNA: 3'- caUCUg-CCG-GC-----UCAGGGCGGuAGCUC- -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 36585 | 0.67 | 0.453851 |
Target: 5'- cUGGcucGCGGCCGGGUguaaCGCCAguUCGAGc -3' miRNA: 3'- cAUC---UGCCGGCUCAgg--GCGGU--AGCUC- -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 32355 | 0.67 | 0.444182 |
Target: 5'- gGUAGGCGuccuuGCCGuGGUCCCuGCCGUUGu- -3' miRNA: 3'- -CAUCUGC-----CGGC-UCAGGG-CGGUAGCuc -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 30955 | 0.67 | 0.444182 |
Target: 5'- -cAGAC--CCGAGUCCUGCCAgCGAc -3' miRNA: 3'- caUCUGccGGCUCAGGGCGGUaGCUc -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 35786 | 0.67 | 0.444182 |
Target: 5'- --cGACGGCCGGuGUCCUuGuCCAUgGGGu -3' miRNA: 3'- cauCUGCCGGCU-CAGGG-C-GGUAgCUC- -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 8483 | 0.67 | 0.444182 |
Target: 5'- -aAGGCcGCCGAGgcgcuggCCCGCCAguaCGAc -3' miRNA: 3'- caUCUGcCGGCUCa------GGGCGGUa--GCUc -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 45594 | 0.68 | 0.43463 |
Target: 5'- -cAGACGG-CGGG-CgCGCCGUCGAc -3' miRNA: 3'- caUCUGCCgGCUCaGgGCGGUAGCUc -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 25632 | 0.68 | 0.43463 |
Target: 5'- -gAGugGGCCGAGgcUCUCGCUgacuUCGGu -3' miRNA: 3'- caUCugCCGGCUC--AGGGCGGu---AGCUc -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 5364 | 0.68 | 0.425199 |
Target: 5'- -gGGAcuCGGCgCGuccGGUCCCGucaCCGUCGAGg -3' miRNA: 3'- caUCU--GCCG-GC---UCAGGGC---GGUAGCUC- -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 9228 | 0.68 | 0.425199 |
Target: 5'- --cGGCGGCgaaGAG-CCCGCCAccaauggCGAGg -3' miRNA: 3'- cauCUGCCGg--CUCaGGGCGGUa------GCUC- -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 14879 | 0.68 | 0.414967 |
Target: 5'- --uGACGGaCGAGcagguguUCgCCGCCAUUGAGg -3' miRNA: 3'- cauCUGCCgGCUC-------AG-GGCGGUAGCUC- -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 44106 | 0.68 | 0.392283 |
Target: 5'- -cGGACGcGCCGAGUCCCugguccuucuccgcgGCCAcgcgcuucUCGGu -3' miRNA: 3'- caUCUGC-CGGCUCAGGG---------------CGGU--------AGCUc -5' |
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11418 | 3' | -58.3 | NC_003085.1 | + | 33220 | 0.69 | 0.379938 |
Target: 5'- -gAGGCacucGGCCGGGcUCCaugaGCCGUUGAGc -3' miRNA: 3'- caUCUG----CCGGCUC-AGGg---CGGUAGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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