miRNA: 3'- -AGGuuC-GCG-CGGGACCU----GAGGua-CUG- -5'
miRNA: 3'- aGGuUC-GCGC-G-GGACCUGAGgUaCUG- -5'
miRNA: 3'- aGGUUCGCGCGgGaCCUGaggUACUG- -5'
miRNA: 3'- aGGuUCGC-GCGGGACC---UGAGGUaCUG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11426 | 5' | -57.9 | NC_003085.1 | + | 34473 | 0.67 | 0.452968 |
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Target: 5'- cUCUggGaCGCuGCCCUGGGgcgcCUCCcagGGCa -3' miRNA: 3'- -AGGuuC-GCG-CGGGACCU----GAGGua-CUG- -5' |
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| 11426 | 5' | -57.9 | NC_003085.1 | + | 30482 | 0.67 | 0.472929 |
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Target: 5'- aCCuGGaCGCGaCgCCUGGACUCgAcGACa -3' miRNA: 3'- aGGuUC-GCGC-G-GGACCUGAGgUaCUG- -5' |
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| 11426 | 5' | -57.9 | NC_003085.1 | + | 26708 | 0.66 | 0.52464 |
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Target: 5'- cUCGAGCGCGCgCaGGGCg--GUGACg -3' miRNA: 3'- aGGUUCGCGCGgGaCCUGaggUACUG- -5' |
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| 11426 | 5' | -57.9 | NC_003085.1 | + | 25235 | 0.74 | 0.171365 |
|
Target: 5'- aCC-GGCGcCGCCCUGGugcGCUCCGcGGCg -3' miRNA: 3'- aGGuUCGC-GCGGGACC---UGAGGUaCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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