Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11429 | 5' | -52.5 | NC_003085.1 | + | 36230 | 0.67 | 0.77313 |
Target: 5'- --cGUCAUCGAGUugCAcguCAuCGcCGCAc -3' miRNA: 3'- guuCAGUAGCUCGugGUu--GU-GC-GCGU- -5' |
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11429 | 5' | -52.5 | NC_003085.1 | + | 31059 | 0.67 | 0.761583 |
Target: 5'- -cAGUCGcuucgccUCGGggugcGCACCcucaGGCACGCGCAg -3' miRNA: 3'- guUCAGU-------AGCU-----CGUGG----UUGUGCGCGU- -5' |
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11429 | 5' | -52.5 | NC_003085.1 | + | 40927 | 0.67 | 0.74879 |
Target: 5'- gCAAGUCAuggcgucaccgccuUCGccuGCGCCcGCugGUGCGc -3' miRNA: 3'- -GUUCAGU--------------AGCu--CGUGGuUGugCGCGU- -5' |
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11429 | 5' | -52.5 | NC_003085.1 | + | 12490 | 0.67 | 0.741243 |
Target: 5'- gGAGUCGaagCG-GCGCCAACAUGgcccCGCGg -3' miRNA: 3'- gUUCAGUa--GCuCGUGGUUGUGC----GCGU- -5' |
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11429 | 5' | -52.5 | NC_003085.1 | + | 12456 | 0.68 | 0.730361 |
Target: 5'- aAGGUCAgcgcUgGGGCGCCAGCA-GUGCc -3' miRNA: 3'- gUUCAGU----AgCUCGUGGUUGUgCGCGu -5' |
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11429 | 5' | -52.5 | NC_003085.1 | + | 38513 | 1.08 | 0.001689 |
Target: 5'- cCAAGUCAUCGAGCACCAACACGCGCAc -3' miRNA: 3'- -GUUCAGUAGCUCGUGGUUGUGCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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