miRNA: 3'- aGAGCU--------UUCGA-GUGa---AGCGGCG-GGGG- -5'
miRNA: 3'- aGA-GCUUUCGAGUGaaGCGGCG--GGGG- -5'
miRNA: 3'- aGAGCuu--UCGAG-----------UGA--------------AGCGGCGGGGG- -5'
miRNA: 3'- -AGAGCUUUCGAGUGAAGCGGCGGGGG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11447 | 5' | -55.7 | NC_003085.1 | + | 36223 | 0.74 | 0.257568 |
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Target: 5'- gCUCGAacgucaucGAGUUgCACgucaUCGCCGCaCCCCg -3' miRNA: 3'- aGAGCU--------UUCGA-GUGa---AGCGGCG-GGGG- -5' |
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| 11447 | 5' | -55.7 | NC_003085.1 | + | 47087 | 0.74 | 0.238315 |
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Target: 5'- cCUgCGAGAGCUCGCg-CGCCGCggCCUCg -3' miRNA: 3'- aGA-GCUUUCGAGUGaaGCGGCG--GGGG- -5' |
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| 11447 | 5' | -55.7 | NC_003085.1 | + | 17387 | 0.74 | 0.236457 |
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Target: 5'- aCUCGccucGGCUCagguccgcauuGCUgucccccaggccacUCGCCGCCCCCa -3' miRNA: 3'- aGAGCuu--UCGAG-----------UGA--------------AGCGGCGGGGG- -5' |
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| 11447 | 5' | -55.7 | NC_003085.1 | + | 30857 | 1.13 | 0.000434 |
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Target: 5'- gUCUCGAAAGCUCACUUCGCCGCCCCCa -3' miRNA: 3'- -AGAGCUUUCGAGUGAAGCGGCGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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