Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11457 | 5' | -61.4 | NC_003085.1 | + | 32842 | 0.72 | 0.166737 |
Target: 5'- gGCCCGAGaggccGCGcGCCGCGGgaccgCCCGg -3' miRNA: 3'- gUGGGCUC-----UGCuCGGCGCCgaa--GGGC- -5' |
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11457 | 5' | -61.4 | NC_003085.1 | + | 35878 | 0.66 | 0.392945 |
Target: 5'- gUACgCGAuGAcacCGGGCCGCGGagUCCCa -3' miRNA: 3'- -GUGgGCU-CU---GCUCGGCGCCgaAGGGc -5' |
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11457 | 5' | -61.4 | NC_003085.1 | + | 41121 | 0.66 | 0.410623 |
Target: 5'- gGCCCaGGcCGAG-CGCGGCacCCCGu -3' miRNA: 3'- gUGGGcUCuGCUCgGCGCCGaaGGGC- -5' |
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11457 | 5' | -61.4 | NC_003085.1 | + | 43506 | 0.66 | 0.410623 |
Target: 5'- aACCagGGGugGAcuggcaucuGCCGCGGCgugUCCGu -3' miRNA: 3'- gUGGg-CUCugCU---------CGGCGCCGaa-GGGC- -5' |
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11457 | 5' | -61.4 | NC_003085.1 | + | 45518 | 0.66 | 0.409727 |
Target: 5'- -cCCCGGGccGCGAcaacaucGCCGCGGCg-CUCGg -3' miRNA: 3'- guGGGCUC--UGCU-------CGGCGCCGaaGGGC- -5' |
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11457 | 5' | -61.4 | NC_003085.1 | + | 46088 | 0.66 | 0.392074 |
Target: 5'- --aCCGAcGGCGAGCCGCugggucggauucuGGCUcaCCCa -3' miRNA: 3'- gugGGCU-CUGCUCGGCG-------------CCGAa-GGGc -5' |
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11457 | 5' | -61.4 | NC_003085.1 | + | 47962 | 0.67 | 0.350972 |
Target: 5'- uGCCCGAgcgccgGACGGGCCGCGucgaGCaagUCgCGg -3' miRNA: 3'- gUGGGCU------CUGCUCGGCGC----CGa--AGgGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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