Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11457 | 5' | -61.4 | NC_003085.1 | + | 24662 | 0.67 | 0.359107 |
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Target: 5'- gCGCgCCGcGACGGGCUgaGCGGCaccaugUUCCGg -3' miRNA: 3'- -GUG-GGCuCUGCUCGG--CGCCGa-----AGGGC- -5' |
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| 11457 | 5' | -61.4 | NC_003085.1 | + | 21563 | 0.66 | 0.375769 |
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Target: 5'- gGCCCGcgagggccauGACGAGuuGCucggugGGCUgCCCGg -3' miRNA: 3'- gUGGGCu---------CUGCUCggCG------CCGAaGGGC- -5' |
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| 11457 | 5' | -61.4 | NC_003085.1 | + | 11361 | 0.66 | 0.392945 |
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Target: 5'- -cUCCGAGACGAugGUgGCGGCg--CCGg -3' miRNA: 3'- guGGGCUCUGCU--CGgCGCCGaagGGC- -5' |
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| 11457 | 5' | -61.4 | NC_003085.1 | + | 45518 | 0.66 | 0.409727 |
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Target: 5'- -cCCCGGGccGCGAcaacaucGCCGCGGCg-CUCGg -3' miRNA: 3'- guGGGCUC--UGCU-------CGGCGCCGaaGGGC- -5' |
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| 11457 | 5' | -61.4 | NC_003085.1 | + | 9087 | 0.66 | 0.410623 |
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Target: 5'- gCGCCaGAGGCGucagcgccgcgGGCagcaGCGGCUUgCCCa -3' miRNA: 3'- -GUGGgCUCUGC-----------UCGg---CGCCGAA-GGGc -5' |
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| 11457 | 5' | -61.4 | NC_003085.1 | + | 43506 | 0.66 | 0.410623 |
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Target: 5'- aACCagGGGugGAcuggcaucuGCCGCGGCgugUCCGu -3' miRNA: 3'- gUGGg-CUCugCU---------CGGCGCCGaa-GGGC- -5' |
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| 11457 | 5' | -61.4 | NC_003085.1 | + | 25390 | 1.07 | 0.000355 |
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Target: 5'- cCACCCGAGACGAGCCGCGGCUUCCCGc -3' miRNA: 3'- -GUGGGCUCUGCUCGGCGCCGAAGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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