miRNA: 3'- -CCaUCaGCGC-GUGGUa-UAGGACCG-GC- -5'
miRNA: 3'- -CCauCAGCGCGUGGuauAgGACCGGC- -5'
miRNA: 3'- -CCAUCa------GCGCGU-GGUauAGGaCCGGc -5'
miRNA: 3'- -CCAUCAGCG--------CGUGGUaua----------GGA-CCGGc -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11465 | 3' | -55.2 | NC_003085.1 | + | 29520 | 0.66 | 0.696728 |
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Target: 5'- aGG-AG-CGCGuCACCAgcGUCUUGGCgCGg -3' miRNA: 3'- -CCaUCaGCGC-GUGGUa-UAGGACCG-GC- -5' |
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| 11465 | 3' | -55.2 | NC_003085.1 | + | 48994 | 0.66 | 0.696728 |
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Target: 5'- uGGccGUCGUGCGCUccuUgUUGGCCGg -3' miRNA: 3'- -CCauCAGCGCGUGGuauAgGACCGGC- -5' |
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| 11465 | 3' | -55.2 | NC_003085.1 | + | 31355 | 0.66 | 0.707567 |
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Target: 5'- cGGUAGgccgccaUGCGCAgCCGcuUCCaGGCCa -3' miRNA: 3'- -CCAUCa------GCGCGU-GGUauAGGaCCGGc -5' |
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| 11465 | 3' | -55.2 | NC_003085.1 | + | 23806 | 0.66 | 0.719402 |
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Target: 5'- cGGUAGUCGCaguccgcaGCACCGaagucagcgagagCCUcGGCCc -3' miRNA: 3'- -CCAUCAGCG--------CGUGGUaua----------GGA-CCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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