miRNA: 3'- cCAU-CAGCGcGUGGUAUaGGACCGGC- -5'
miRNA: 3'- -CCAUcaGCGCGUGGUa-UAGGACCGgc -5'
miRNA: 3'- -CCAUCA--GCGCGuGGUa-----UAGGACCGGc -5'
miRNA: 3'- -CCAUCAGCGCGUGGUAUAGGACCGGC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11465 | 3' | -55.2 | NC_003085.1 | + | 10231 | 0.72 | 0.357674 |
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Target: 5'- aGUGcGUUGUaCGCCGUAgCCUGGCCGa -3' miRNA: 3'- cCAU-CAGCGcGUGGUAUaGGACCGGC- -5' |
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| 11465 | 3' | -55.2 | NC_003085.1 | + | 23003 | 0.72 | 0.349203 |
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Target: 5'- aGGUG--CGCGCGCCGcaGUCCUGGCg- -3' miRNA: 3'- -CCAUcaGCGCGUGGUa-UAGGACCGgc -5' |
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| 11465 | 3' | -55.2 | NC_003085.1 | + | 21273 | 0.78 | 0.160041 |
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Target: 5'- aGGUGGUgcCGCGC-CCAgacuucGUCCUGGCCc -3' miRNA: 3'- -CCAUCA--GCGCGuGGUa-----UAGGACCGGc -5' |
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| 11465 | 3' | -55.2 | NC_003085.1 | + | 23227 | 1.11 | 0.000652 |
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Target: 5'- uGGUAGUCGCGCACCAUAUCCUGGCCGa -3' miRNA: 3'- -CCAUCAGCGCGUGGUAUAGGACCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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