miRNA: 3'- cUGCCGugUUGa-CGUGCUCCAACUGU- -5'
miRNA: 3'- cuGCCGUGuUGACGuGCUCCAACUgu -5'
miRNA: 3'- cUGcCGUGU----------UGACGUGCUCC--AACUGU- -5'
miRNA: 3'- -CUGCCGUGUugaCGUGCUCCaACUGU- -5'
miRNA: 3'- -CUGCCGUGUUGACGUGCUCCAACUGU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11465 | 5' | -53.1 | NC_003085.1 | + | 12017 | 0.72 | 0.431058 |
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Target: 5'- uGCGGU--GGCggGCGCGGGGUUGGCGc -3' miRNA: 3'- cUGCCGugUUGa-CGUGCUCCAACUGU- -5' |
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| 11465 | 5' | -53.1 | NC_003085.1 | + | 18345 | 0.73 | 0.392554 |
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Target: 5'- -uCGGCGC-GCUGCuCGGGGUUGAg- -3' miRNA: 3'- cuGCCGUGuUGACGuGCUCCAACUgu -5' |
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| 11465 | 5' | -53.1 | NC_003085.1 | + | 3890 | 0.74 | 0.356357 |
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Target: 5'- uGCaGCGCAuugaggccgcGCUGCGCGAGGagUUGACAc -3' miRNA: 3'- cUGcCGUGU----------UGACGUGCUCC--AACUGU- -5' |
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| 11465 | 5' | -53.1 | NC_003085.1 | + | 33218 | 0.78 | 0.179247 |
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Target: 5'- cGGCGGCGCA---GCACGAGGaUGACGa -3' miRNA: 3'- -CUGCCGUGUugaCGUGCUCCaACUGU- -5' |
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| 11465 | 5' | -53.1 | NC_003085.1 | + | 23266 | 1.09 | 0.001374 |
|
Target: 5'- aGACGGCACAACUGCACGAGGUUGACAu -3' miRNA: 3'- -CUGCCGUGUUGACGUGCUCCAACUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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