Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11483 | 5' | -56.1 | NC_003085.1 | + | 6376 | 0.68 | 0.493908 |
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Target: 5'- --cUGGC-GGCCGGucguuucUUCCUcgGCCg -3' miRNA: 3'- aaaACCGaCCGGCCuu-----AGGGGuaCGG- -5' |
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| 11483 | 5' | -56.1 | NC_003085.1 | + | 19370 | 0.67 | 0.519401 |
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Target: 5'- ---aGGC-GGCCGGcguccuucugagccaGcaaGUCCuCCAUGCCg -3' miRNA: 3'- aaaaCCGaCCGGCC---------------U---UAGG-GGUACGG- -5' |
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| 11483 | 5' | -56.1 | NC_003085.1 | + | 7291 | 0.67 | 0.525857 |
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Target: 5'- ---aGGCUGGCUgcgacgGGAGagUCCAUGUCc -3' miRNA: 3'- aaaaCCGACCGG------CCUUagGGGUACGG- -5' |
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| 11483 | 5' | -56.1 | NC_003085.1 | + | 573 | 0.67 | 0.536681 |
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Target: 5'- ---cGGCUGGCgGGccaaugCUCCGUGUg -3' miRNA: 3'- aaaaCCGACCGgCCuua---GGGGUACGg -5' |
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| 11483 | 5' | -56.1 | NC_003085.1 | + | 20621 | 0.67 | 0.558552 |
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Target: 5'- ---gGGC-GGCgCGGGugcuugcuUCCCCAUGgCCu -3' miRNA: 3'- aaaaCCGaCCG-GCCUu-------AGGGGUAC-GG- -5' |
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| 11483 | 5' | -56.1 | NC_003085.1 | + | 43782 | 0.66 | 0.580667 |
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Target: 5'- ---gGGUcagcaucccgaaUGGCCGGAAUCUCUccGCg -3' miRNA: 3'- aaaaCCG------------ACCGGCCUUAGGGGuaCGg -5' |
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| 11483 | 5' | -56.1 | NC_003085.1 | + | 38717 | 0.66 | 0.590681 |
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Target: 5'- ---cGGCgcccgugUGGCCGGAcagcaacagcGUCCgCuUGCCg -3' miRNA: 3'- aaaaCCG-------ACCGGCCU----------UAGGgGuACGG- -5' |
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| 11483 | 5' | -56.1 | NC_003085.1 | + | 26611 | 0.66 | 0.614149 |
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Target: 5'- ---gGGCgcuGCCGGAcUCUCCuucgcUGCCg -3' miRNA: 3'- aaaaCCGac-CGGCCUuAGGGGu----ACGG- -5' |
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| 11483 | 5' | -56.1 | NC_003085.1 | + | 15046 | 1.1 | 0.000482 |
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Target: 5'- gUUUUGGCUGGCCGGAAUCCCCAUGCCg -3' miRNA: 3'- -AAAACCGACCGGCCUUAGGGGUACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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