Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11490 | 3' | -53.6 | NC_003085.1 | + | 1226 | 0.69 | 0.613575 |
Target: 5'- -cGCGCUgcUGgagGAGUUGGAGgGGCUGCg -3' miRNA: 3'- guCGUGA--GCa--CUUGACCUCgCUGACG- -5' |
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11490 | 3' | -53.6 | NC_003085.1 | + | 15176 | 0.68 | 0.624829 |
Target: 5'- -cGaCGCUCGgcgGAACacaaGGGCGGCUGCu -3' miRNA: 3'- guC-GUGAGCa--CUUGac--CUCGCUGACG- -5' |
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11490 | 3' | -53.6 | NC_003085.1 | + | 5858 | 0.68 | 0.658588 |
Target: 5'- -uGCACUCG-GGA-UGGcAGCGcCUGCg -3' miRNA: 3'- guCGUGAGCaCUUgACC-UCGCuGACG- -5' |
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11490 | 3' | -53.6 | NC_003085.1 | + | 37756 | 0.66 | 0.767656 |
Target: 5'- uCGGCguccuccuGCUCGcccaUGAACUGGuAGCGGCa-- -3' miRNA: 3'- -GUCG--------UGAGC----ACUUGACC-UCGCUGacg -5' |
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11490 | 3' | -53.6 | NC_003085.1 | + | 12360 | 1.13 | 0.000693 |
Target: 5'- cCAGCACUCGUGAACUGGAGCGACUGCg -3' miRNA: 3'- -GUCGUGAGCACUUGACCUCGCUGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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