miRNA: 3'- cACC-GUAGUGA-GGGGa-----CAGCGGGUCg -5'
miRNA: 3'- caCCGUAgUGAG--GGGAc-AGCGGGUCG- -5'
miRNA: 3'- -CACCGuaguGAGGGGACAGCGGGuCG- -5'
miRNA: 3'- -CACCGUAGUGAGGGGACAGCGGGUCG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11502 | 3' | -58.9 | NC_003085.1 | + | 29716 | 0.71 | 0.215171 |
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Target: 5'- cUGGuCGUCGCUaCCUCggcgucGUCGCCCAGg -3' miRNA: 3'- cACC-GUAGUGA-GGGGa-----CAGCGGGUCg -5' |
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| 11502 | 3' | -58.9 | NC_003085.1 | + | 46611 | 0.74 | 0.136162 |
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Target: 5'- -cGGCAUgGCUCagCCCUccUCGCCCGGCu -3' miRNA: 3'- caCCGUAgUGAG--GGGAc-AGCGGGUCG- -5' |
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| 11502 | 3' | -58.9 | NC_003085.1 | + | 31189 | 0.78 | 0.079956 |
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Target: 5'- gGUGGCuu--CUUCCCUGUCGCCCuGUg -3' miRNA: 3'- -CACCGuaguGAGGGGACAGCGGGuCG- -5' |
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| 11502 | 3' | -58.9 | NC_003085.1 | + | 7253 | 1.12 | 0.000222 |
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Target: 5'- aGUGGCAUCACUCCCCUGUCGCCCAGCg -3' miRNA: 3'- -CACCGUAGUGAGGGGACAGCGGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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