Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11509 | 5' | -59.1 | NC_003085.1 | + | 39118 | 0.67 | 0.436962 |
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Target: 5'- uCCGCGgugCCUCCCgggagGCGACgugGCg- -3' miRNA: 3'- -GGCGCa--GGAGGGagg--CGCUGaa-CGag -5' |
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| 11509 | 5' | -59.1 | NC_003085.1 | + | 40900 | 0.72 | 0.229818 |
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Target: 5'- gCCGCGaCCUCaagaCCUgCGUGACgaGCUCg -3' miRNA: 3'- -GGCGCaGGAG----GGAgGCGCUGaaCGAG- -5' |
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| 11509 | 5' | -59.1 | NC_003085.1 | + | 41036 | 0.67 | 0.427578 |
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Target: 5'- -gGCGUCCUCCUgg-GCGGCUUgGCg- -3' miRNA: 3'- ggCGCAGGAGGGaggCGCUGAA-CGag -5' |
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| 11509 | 5' | -59.1 | NC_003085.1 | + | 42135 | 0.76 | 0.105935 |
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Target: 5'- cCCGCGUCCUCagccgCCUCCGCGAUg-GCcgUCg -3' miRNA: 3'- -GGCGCAGGAG-----GGAGGCGCUGaaCG--AG- -5' |
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| 11509 | 5' | -59.1 | NC_003085.1 | + | 44239 | 0.69 | 0.348815 |
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Target: 5'- gCCGCuUCCUgCCgggCCGCGuCgcGCUCg -3' miRNA: 3'- -GGCGcAGGAgGGa--GGCGCuGaaCGAG- -5' |
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| 11509 | 5' | -59.1 | NC_003085.1 | + | 48510 | 0.66 | 0.495601 |
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Target: 5'- gCUGCGaCCUCCUUCUcgGCGGCgaggGCg- -3' miRNA: 3'- -GGCGCaGGAGGGAGG--CGCUGaa--CGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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