Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11509 | 5' | -59.1 | NC_003085.1 | + | 25809 | 0.69 | 0.332776 |
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Target: 5'- gCgGCGUUCg-CCUgCGCGGCcUGCUCg -3' miRNA: 3'- -GgCGCAGGagGGAgGCGCUGaACGAG- -5' |
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| 11509 | 5' | -59.1 | NC_003085.1 | + | 5300 | 0.68 | 0.391273 |
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Target: 5'- aCCGaagucgCCUCCCUCCGCGAgc-GCg- -3' miRNA: 3'- -GGCgca---GGAGGGAGGCGCUgaaCGag -5' |
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| 11509 | 5' | -59.1 | NC_003085.1 | + | 23585 | 0.67 | 0.418315 |
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Target: 5'- gCGCGUCCggcaCggCgGCGAgUUGCUCg -3' miRNA: 3'- gGCGCAGGag--GgaGgCGCUgAACGAG- -5' |
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| 11509 | 5' | -59.1 | NC_003085.1 | + | 39118 | 0.67 | 0.436962 |
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Target: 5'- uCCGCGgugCCUCCCgggagGCGACgugGCg- -3' miRNA: 3'- -GGCGCa--GGAGGGagg--CGCUGaa-CGag -5' |
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| 11509 | 5' | -59.1 | NC_003085.1 | + | 25531 | 0.66 | 0.515938 |
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Target: 5'- -aGCGccCCUCgCUUCGUGAggugucCUUGCUCg -3' miRNA: 3'- ggCGCa-GGAGgGAGGCGCU------GAACGAG- -5' |
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| 11509 | 5' | -59.1 | NC_003085.1 | + | 1472 | 1.11 | 0.000282 |
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Target: 5'- gCCGCGUCCUCCCUCCGCGACUUGCUCg -3' miRNA: 3'- -GGCGCAGGAGGGAGGCGCUGAACGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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