Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1154 | 3' | -53.6 | NC_001132.2 | + | 3722 | 1.14 | 0.00372 |
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Target: 5'- cCCGAUCCCAAGAAGGACGACGACCGGg -3' miRNA: 3'- -GGCUAGGGUUCUUCCUGCUGCUGGCC- -5' |
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| 1154 | 3' | -53.6 | NC_001132.2 | + | 15939 | 0.8 | 0.427318 |
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Target: 5'- aCGAuccgccaacaucUCCCAAGGAGGACGugGAgCGa -3' miRNA: 3'- gGCU------------AGGGUUCUUCCUGCugCUgGCc -5' |
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| 1154 | 3' | -53.6 | NC_001132.2 | + | 1928 | 0.75 | 0.638598 |
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Target: 5'- gCGAcaguaaucaUCCCGAGGAGGuCGACGACUucguGGa -3' miRNA: 3'- gGCU---------AGGGUUCUUCCuGCUGCUGG----CC- -5' |
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| 1154 | 3' | -53.6 | NC_001132.2 | + | 15644 | 0.71 | 0.874221 |
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Target: 5'- uUGggCCCc-GggGGACGACGcCUGGa -3' miRNA: 3'- gGCuaGGGuuCuuCCUGCUGCuGGCC- -5' |
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| 1154 | 3' | -53.6 | NC_001132.2 | + | 91764 | 0.69 | 0.91881 |
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Target: 5'- cCCcauGUCCCGcgaAGAAagcgucGGACGACGACCa- -3' miRNA: 3'- -GGc--UAGGGU---UCUU------CCUGCUGCUGGcc -5' |
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| 1154 | 3' | -53.6 | NC_001132.2 | + | 158281 | 0.68 | 0.952218 |
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Target: 5'- -gGGUUCCuugggaauGGggGGACaauCGGCCGGg -3' miRNA: 3'- ggCUAGGGu-------UCuuCCUGcu-GCUGGCC- -5' |
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| 1154 | 3' | -53.6 | NC_001132.2 | + | 10851 | 0.67 | 0.974857 |
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Target: 5'- aUCGGUCCUGGaucuGAGGGACGcccuccagaGACUGGg -3' miRNA: 3'- -GGCUAGGGUU----CUUCCUGCug-------CUGGCC- -5' |
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| 1154 | 3' | -53.6 | NC_001132.2 | + | 154341 | 0.66 | 0.979563 |
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Target: 5'- aCGAUCUCGu--AGGACGcucguagaaccACGACCGc -3' miRNA: 3'- gGCUAGGGUucuUCCUGC-----------UGCUGGCc -5' |
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| 1154 | 3' | -53.6 | NC_001132.2 | + | 48215 | 0.66 | 0.986969 |
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Target: 5'- cCCGuUCCCAcGAAGGuacacACGuacuACGACuCGGc -3' miRNA: 3'- -GGCuAGGGUuCUUCC-----UGC----UGCUG-GCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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