miRNA display CGI


Results 1 - 9 of 9 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1154 3' -53.6 NC_001132.2 + 1928 0.75 0.638598
Target:  5'- gCGAcaguaaucaUCCCGAGGAGGuCGACGACUucguGGa -3'
miRNA:   3'- gGCU---------AGGGUUCUUCCuGCUGCUGG----CC- -5'
1154 3' -53.6 NC_001132.2 + 3722 1.14 0.00372
Target:  5'- cCCGAUCCCAAGAAGGACGACGACCGGg -3'
miRNA:   3'- -GGCUAGGGUUCUUCCUGCUGCUGGCC- -5'
1154 3' -53.6 NC_001132.2 + 10851 0.67 0.974857
Target:  5'- aUCGGUCCUGGaucuGAGGGACGcccuccagaGACUGGg -3'
miRNA:   3'- -GGCUAGGGUU----CUUCCUGCug-------CUGGCC- -5'
1154 3' -53.6 NC_001132.2 + 15644 0.71 0.874221
Target:  5'- uUGggCCCc-GggGGACGACGcCUGGa -3'
miRNA:   3'- gGCuaGGGuuCuuCCUGCUGCuGGCC- -5'
1154 3' -53.6 NC_001132.2 + 15939 0.8 0.427318
Target:  5'- aCGAuccgccaacaucUCCCAAGGAGGACGugGAgCGa -3'
miRNA:   3'- gGCU------------AGGGUUCUUCCUGCugCUgGCc -5'
1154 3' -53.6 NC_001132.2 + 48215 0.66 0.986969
Target:  5'- cCCGuUCCCAcGAAGGuacacACGuacuACGACuCGGc -3'
miRNA:   3'- -GGCuAGGGUuCUUCC-----UGC----UGCUG-GCC- -5'
1154 3' -53.6 NC_001132.2 + 91764 0.69 0.91881
Target:  5'- cCCcauGUCCCGcgaAGAAagcgucGGACGACGACCa- -3'
miRNA:   3'- -GGc--UAGGGU---UCUU------CCUGCUGCUGGcc -5'
1154 3' -53.6 NC_001132.2 + 154341 0.66 0.979563
Target:  5'- aCGAUCUCGu--AGGACGcucguagaaccACGACCGc -3'
miRNA:   3'- gGCUAGGGUucuUCCUGC-----------UGCUGGCc -5'
1154 3' -53.6 NC_001132.2 + 158281 0.68 0.952218
Target:  5'- -gGGUUCCuugggaauGGggGGACaauCGGCCGGg -3'
miRNA:   3'- ggCUAGGGu-------UCuuCCUGcu-GCUGGCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.