miRNA display CGI


Results 21 - 26 of 26 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
11556 3' -57.2 NC_003085.1 + 37328 0.66 0.564391
Target:  5'- -aGGUaCGUGUGGuCUGCGaCGCCugccuugagcgcGGCGg -3'
miRNA:   3'- gaCCAaGUACGCC-GAUGC-GUGG------------CCGC- -5'
11556 3' -57.2 NC_003085.1 + 34927 0.66 0.564391
Target:  5'- gUGGUgcaggcgCAcGCGGCggaguUGCugGCCGGCGg -3'
miRNA:   3'- gACCAa------GUaCGCCGau---GCG--UGGCCGC- -5'
11556 3' -57.2 NC_003085.1 + 39255 0.66 0.564391
Target:  5'- uCUGGgccgcagCAUGU-GCUGCGCGCCuccucuacGGCGc -3'
miRNA:   3'- -GACCaa-----GUACGcCGAUGCGUGG--------CCGC- -5'
11556 3' -57.2 NC_003085.1 + 34518 0.66 0.586074
Target:  5'- ----cUCAUGCuGGCcgACGuCGCCGGCu -3'
miRNA:   3'- gaccaAGUACG-CCGa-UGC-GUGGCCGc -5'
11556 3' -57.2 NC_003085.1 + 23877 0.66 0.596977
Target:  5'- -cGGUgc-UGCGGaCUGCGacUACCGGCc -3'
miRNA:   3'- gaCCAaguACGCC-GAUGC--GUGGCCGc -5'
11556 3' -57.2 NC_003085.1 + 39597 0.66 0.596977
Target:  5'- gCUGGagcgCGUcGCGGa-GCGCACCGaGCa -3'
miRNA:   3'- -GACCaa--GUA-CGCCgaUGCGUGGC-CGc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.