Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11556 | 3' | -57.2 | NC_003085.1 | + | 37328 | 0.66 | 0.564391 |
Target: 5'- -aGGUaCGUGUGGuCUGCGaCGCCugccuugagcgcGGCGg -3' miRNA: 3'- gaCCAaGUACGCC-GAUGC-GUGG------------CCGC- -5' |
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11556 | 3' | -57.2 | NC_003085.1 | + | 34927 | 0.66 | 0.564391 |
Target: 5'- gUGGUgcaggcgCAcGCGGCggaguUGCugGCCGGCGg -3' miRNA: 3'- gACCAa------GUaCGCCGau---GCG--UGGCCGC- -5' |
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11556 | 3' | -57.2 | NC_003085.1 | + | 39255 | 0.66 | 0.564391 |
Target: 5'- uCUGGgccgcagCAUGU-GCUGCGCGCCuccucuacGGCGc -3' miRNA: 3'- -GACCaa-----GUACGcCGAUGCGUGG--------CCGC- -5' |
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11556 | 3' | -57.2 | NC_003085.1 | + | 34518 | 0.66 | 0.586074 |
Target: 5'- ----cUCAUGCuGGCcgACGuCGCCGGCu -3' miRNA: 3'- gaccaAGUACG-CCGa-UGC-GUGGCCGc -5' |
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11556 | 3' | -57.2 | NC_003085.1 | + | 23877 | 0.66 | 0.596977 |
Target: 5'- -cGGUgc-UGCGGaCUGCGacUACCGGCc -3' miRNA: 3'- gaCCAaguACGCC-GAUGC--GUGGCCGc -5' |
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11556 | 3' | -57.2 | NC_003085.1 | + | 39597 | 0.66 | 0.596977 |
Target: 5'- gCUGGagcgCGUcGCGGa-GCGCACCGaGCa -3' miRNA: 3'- -GACCaa--GUA-CGCCgaUGCGUGGC-CGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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