Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11572 | 3' | -61 | NC_003085.1 | + | 23791 | 0.67 | 0.323504 |
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Target: 5'- cGGUGGGggccgggGCCGUcuugacggaggaggcUGCUgcgCGGGCCcGCUGc -3' miRNA: 3'- -UCGCCUa------CGGCA---------------ACGA---GCCCGG-CGAC- -5' |
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| 11572 | 3' | -61 | NC_003085.1 | + | 4039 | 0.67 | 0.343273 |
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Target: 5'- aAGCGcg-GCCGUcugGCUccaucccCGGGCCGCg- -3' miRNA: 3'- -UCGCcuaCGGCAa--CGA-------GCCCGGCGac -5' |
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| 11572 | 3' | -61 | NC_003085.1 | + | 10793 | 0.66 | 0.351421 |
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Target: 5'- cAGCGuccgcuUGCCGUcagccagUGCcucgCGGGCCGCg- -3' miRNA: 3'- -UCGCcu----ACGGCA-------ACGa---GCCCGGCGac -5' |
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| 11572 | 3' | -61 | NC_003085.1 | + | 37111 | 0.66 | 0.368974 |
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Target: 5'- cGCGGuacagGCCGgUGUcCGGGUCGCc- -3' miRNA: 3'- uCGCCua---CGGCaACGaGCCCGGCGac -5' |
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| 11572 | 3' | -61 | NC_003085.1 | + | 22862 | 0.66 | 0.377541 |
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Target: 5'- gGGCGGGagcgGCCGUUGC-CGcuGCCGUc- -3' miRNA: 3'- -UCGCCUa---CGGCAACGaGCc-CGGCGac -5' |
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| 11572 | 3' | -61 | NC_003085.1 | + | 44943 | 0.75 | 0.093039 |
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Target: 5'- uGCGGAUGCgGUUGCgaGGGCC-CUGc -3' miRNA: 3'- uCGCCUACGgCAACGagCCCGGcGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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