Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11572 | 5' | -55 | NC_003085.1 | + | 46747 | 0.67 | 0.641364 |
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Target: 5'- cGGCGGc-GCUCCAGGcgGC-GGCGaagcgCCu -3' miRNA: 3'- -CCGUCaaCGAGGUCCa-CGaCUGCa----GG- -5' |
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| 11572 | 5' | -55 | NC_003085.1 | + | 43556 | 0.67 | 0.663527 |
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Target: 5'- aGGCGG-UGggCCguuugucgaguGGGUGCgUGACGUCa -3' miRNA: 3'- -CCGUCaACgaGG-----------UCCACG-ACUGCAGg -5' |
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| 11572 | 5' | -55 | NC_003085.1 | + | 45764 | 0.66 | 0.674569 |
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Target: 5'- cGCAG-UGCUCCGugacucgcgGCUGACGgacgCCg -3' miRNA: 3'- cCGUCaACGAGGUcca------CGACUGCa---GG- -5' |
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| 11572 | 5' | -55 | NC_003085.1 | + | 32438 | 0.66 | 0.693242 |
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Target: 5'- cGCAGcUGCgcucUCCAGGcguuugaaagccccUGCgGugGUCCc -3' miRNA: 3'- cCGUCaACG----AGGUCC--------------ACGaCugCAGG- -5' |
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| 11572 | 5' | -55 | NC_003085.1 | + | 46389 | 0.66 | 0.718217 |
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Target: 5'- gGGCAGUugaaguUGCUCUGGGa--UGACGUg- -3' miRNA: 3'- -CCGUCA------ACGAGGUCCacgACUGCAgg -5' |
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| 11572 | 5' | -55 | NC_003085.1 | + | 21414 | 0.65 | 0.727873 |
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Target: 5'- uGGCGc--GCUCCAGcagggugaugcgcGUGCUGACGaggucgauuUCCu -3' miRNA: 3'- -CCGUcaaCGAGGUC-------------CACGACUGC---------AGG- -5' |
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| 11572 | 5' | -55 | NC_003085.1 | + | 6762 | 0.73 | 0.307592 |
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Target: 5'- gGGCGGccgcaagccGCUCCAGGgccaucagGgaGGCGUCCa -3' miRNA: 3'- -CCGUCaa-------CGAGGUCCa-------CgaCUGCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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