Results 21 - 37 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1159 | 3' | -47.4 | NC_001132.2 | + | 56075 | 0.68 | 0.99926 |
Target: 5'- -aACGGAAUGcuGGAUAGAacucuCGUCCCUg -3' miRNA: 3'- ugUGCUUUGCucUUUAUCU-----GCAGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 21444 | 0.68 | 0.999401 |
Target: 5'- gACACGAAGCGAuGAGUuuuauacgacAGGCGauccguUCCUCg -3' miRNA: 3'- -UGUGCUUUGCUcUUUA----------UCUGC------AGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 154476 | 0.68 | 0.999401 |
Target: 5'- cGCGCGuAGACGuc--GUAGACGUCgUCCu -3' miRNA: 3'- -UGUGC-UUUGCucuuUAUCUGCAG-GGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 106741 | 0.68 | 0.99926 |
Target: 5'- uACACGAAuCGaAGAAAUucGACGUCUa- -3' miRNA: 3'- -UGUGCUUuGC-UCUUUAu-CUGCAGGgg -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 39913 | 0.69 | 0.998367 |
Target: 5'- uACACGGuauccGACGAGuuacUGGACGUUCUa -3' miRNA: 3'- -UGUGCU-----UUGCUCuuu-AUCUGCAGGGg -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 6241 | 0.69 | 0.998038 |
Target: 5'- aACGCGAuGGCGccAGAUGGACGauaUCCCUu -3' miRNA: 3'- -UGUGCU-UUGCucUUUAUCUGC---AGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 140202 | 0.69 | 0.997613 |
Target: 5'- gACAUGAGACGgacgaucGGAGGUAG-CGUCgUCg -3' miRNA: 3'- -UGUGCUUUGC-------UCUUUAUCuGCAGgGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 103345 | 0.69 | 0.99721 |
Target: 5'- uACAUGAuuAugGAcaagGAGAUAGAaggguguugcaCGUCCCCc -3' miRNA: 3'- -UGUGCU--UugCU----CUUUAUCU-----------GCAGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 43114 | 0.7 | 0.996699 |
Target: 5'- uACACGGAACGAu---UGGAU-UCCCCc -3' miRNA: 3'- -UGUGCUUUGCUcuuuAUCUGcAGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 117425 | 0.7 | 0.994681 |
Target: 5'- cGCugGGAuUGAGAAugAGAUgauaGUCCCCu -3' miRNA: 3'- -UGugCUUuGCUCUUuaUCUG----CAGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 11575 | 0.71 | 0.989203 |
Target: 5'- uGCGCGuguCGAGAuaucggacuAUGGACGUUCCg -3' miRNA: 3'- -UGUGCuuuGCUCUu--------UAUCUGCAGGGg -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 56874 | 0.71 | 0.987708 |
Target: 5'- aACGCGGAugGAGAAu--GuACGUCCa- -3' miRNA: 3'- -UGUGCUUugCUCUUuauC-UGCAGGgg -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 11258 | 0.73 | 0.975154 |
Target: 5'- gUACGuuaaggauaAGACGGGAGG-AGACGUCCCa -3' miRNA: 3'- uGUGC---------UUUGCUCUUUaUCUGCAGGGg -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 86456 | 0.73 | 0.969392 |
Target: 5'- uUugGuAGAgGAGucGUAGACGUCCUCa -3' miRNA: 3'- uGugC-UUUgCUCuuUAUCUGCAGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 9056 | 0.75 | 0.926274 |
Target: 5'- uUugGAAACGAGcGAacGACGUCUCCu -3' miRNA: 3'- uGugCUUUGCUCuUUauCUGCAGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 34504 | 0.81 | 0.674617 |
Target: 5'- -aGCGAAACGAGAcg-AGACGUUUCCa -3' miRNA: 3'- ugUGCUUUGCUCUuuaUCUGCAGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 11452 | 1.14 | 0.012002 |
Target: 5'- aACACGAAACGAGAAAUAGACGUCCCCg -3' miRNA: 3'- -UGUGCUUUGCUCUUUAUCUGCAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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