Results 21 - 37 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1159 | 3' | -47.4 | NC_001132.2 | + | 2020 | 0.66 | 0.999935 |
Target: 5'- -gACG-AGCGAcGggGUcGGCGUCCUa -3' miRNA: 3'- ugUGCuUUGCU-CuuUAuCUGCAGGGg -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 8316 | 0.66 | 0.999935 |
Target: 5'- uCGCGAAGaGAGAAGUca--GUCCCUa -3' miRNA: 3'- uGUGCUUUgCUCUUUAucugCAGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 22133 | 0.66 | 0.999914 |
Target: 5'- -aACGuc-CG-GAAAUAGACGUauaCCCg -3' miRNA: 3'- ugUGCuuuGCuCUUUAUCUGCAg--GGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 12258 | 0.66 | 0.999914 |
Target: 5'- gGCAacccgGAAugGAGGAAUGGcACGacgCUCCu -3' miRNA: 3'- -UGUg----CUUugCUCUUUAUC-UGCa--GGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 8349 | 0.66 | 0.999888 |
Target: 5'- --cCGAGGCGGGu-GUGGaggucuGCGUUCCCg -3' miRNA: 3'- uguGCUUUGCUCuuUAUC------UGCAGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 77548 | 0.66 | 0.999851 |
Target: 5'- uCGCGA--UGAGAGuaugcaucguaguGUAGACGUCCg- -3' miRNA: 3'- uGUGCUuuGCUCUU-------------UAUCUGCAGGgg -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 106460 | 0.67 | 0.999812 |
Target: 5'- -gACGAu-CGuGuuGUAGugGUCCCg -3' miRNA: 3'- ugUGCUuuGCuCuuUAUCugCAGGGg -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 72879 | 0.67 | 0.999812 |
Target: 5'- uCAuCGGuuuguACGGGGuuggGGACGUCCUCg -3' miRNA: 3'- uGU-GCUu----UGCUCUuua-UCUGCAGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 63568 | 0.66 | 0.999951 |
Target: 5'- -gGCGuuuUGAGAAuu-GGCGaUCCCCg -3' miRNA: 3'- ugUGCuuuGCUCUUuauCUGC-AGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 159650 | 0.67 | 0.999695 |
Target: 5'- cCACGcugauguaguuAAACGAcGAGGUAcACGUCUCCg -3' miRNA: 3'- uGUGC-----------UUUGCU-CUUUAUcUGCAGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 23980 | 0.67 | 0.999695 |
Target: 5'- --gUGAGACGAuGGAUgcGGACGUCgCCg -3' miRNA: 3'- uguGCUUUGCUcUUUA--UCUGCAGgGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 2427 | 0.67 | 0.999607 |
Target: 5'- -aACGAAgugucccGCGGGguAUGG-CGUCUCCg -3' miRNA: 3'- ugUGCUU-------UGCUCuuUAUCuGCAGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 29544 | 0.67 | 0.999519 |
Target: 5'- -uGCGAGACGAcGAcc----CGUCCCCg -3' miRNA: 3'- ugUGCUUUGCU-CUuuaucuGCAGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 119195 | 0.67 | 0.999519 |
Target: 5'- uUugGAuAGCGGGGAc--GGCGUCUCCu -3' miRNA: 3'- uGugCU-UUGCUCUUuauCUGCAGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 92467 | 0.67 | 0.999508 |
Target: 5'- cCGCGAGaaacaccGCGAcgGAGGaAGACGaCCCCa -3' miRNA: 3'- uGUGCUU-------UGCU--CUUUaUCUGCaGGGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 154476 | 0.68 | 0.999401 |
Target: 5'- cGCGCGuAGACGuc--GUAGACGUCgUCCu -3' miRNA: 3'- -UGUGC-UUUGCucuuUAUCUGCAG-GGG- -5' |
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1159 | 3' | -47.4 | NC_001132.2 | + | 21444 | 0.68 | 0.999401 |
Target: 5'- gACACGAAGCGAuGAGUuuuauacgacAGGCGauccguUCCUCg -3' miRNA: 3'- -UGUGCUUUGCUcUUUA----------UCUGC------AGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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