Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11595 | 5' | -59 | NC_003085.1 | + | 28605 | 0.67 | 0.447508 |
Target: 5'- cACGC-CGGuGGUGUgGCGCUcgcugcugcggccgGCCUuucgauaCCCCu -3' miRNA: 3'- -UGCGaGCC-CCAUA-CGCGA--------------UGGA-------GGGG- -5' |
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11595 | 5' | -59 | NC_003085.1 | + | 10775 | 0.67 | 0.451367 |
Target: 5'- gACGCUgGGGGcAUGC-C-ACCUCUUCc -3' miRNA: 3'- -UGCGAgCCCCaUACGcGaUGGAGGGG- -5' |
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11595 | 5' | -59 | NC_003085.1 | + | 20606 | 0.66 | 0.461093 |
Target: 5'- aGCGCcgcggaagUCGGGcGgcgcggGUGCuUGCUUCCCCa -3' miRNA: 3'- -UGCG--------AGCCC-Caua---CGCG-AUGGAGGGG- -5' |
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11595 | 5' | -59 | NC_003085.1 | + | 14680 | 0.66 | 0.490911 |
Target: 5'- cCGCUCGucGUAcUGCGcCUGCCgaaCCCg -3' miRNA: 3'- uGCGAGCccCAU-ACGC-GAUGGag-GGG- -5' |
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11595 | 5' | -59 | NC_003085.1 | + | 33877 | 0.66 | 0.511278 |
Target: 5'- -gGCggCGGGGUuUGCG--ACCUCCUg -3' miRNA: 3'- ugCGa-GCCCCAuACGCgaUGGAGGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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