Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11595 | 5' | -59 | NC_003085.1 | + | 36733 | 1.11 | 0.000291 |
Target: 5'- gACGCUCGGGGUAUGCGCUACCUCCCCu -3' miRNA: 3'- -UGCGAGCCCCAUACGCGAUGGAGGGG- -5' |
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11595 | 5' | -59 | NC_003085.1 | + | 37354 | 0.68 | 0.395507 |
Target: 5'- gGC-CUCGGGGgcguccgacuUGCGCgcgucACCcgCCCCg -3' miRNA: 3'- -UGcGAGCCCCau--------ACGCGa----UGGa-GGGG- -5' |
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11595 | 5' | -59 | NC_003085.1 | + | 42183 | 0.67 | 0.404505 |
Target: 5'- -aGCUUGGcGGcgGUGCGCcACgUCUCCu -3' miRNA: 3'- ugCGAGCC-CCa-UACGCGaUGgAGGGG- -5' |
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11595 | 5' | -59 | NC_003085.1 | + | 45972 | 0.71 | 0.231918 |
Target: 5'- uCGcCUUGGGGcggcagAUGCGCgccUCUCCCCa -3' miRNA: 3'- uGC-GAGCCCCa-----UACGCGau-GGAGGGG- -5' |
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11595 | 5' | -59 | NC_003085.1 | + | 48001 | 0.69 | 0.336253 |
Target: 5'- gGCGaCUUGGGGUgucAUGCGC----UCCCCu -3' miRNA: 3'- -UGC-GAGCCCCA---UACGCGauggAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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