Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11597 | 3' | -55.3 | NC_003085.1 | + | 34420 | 0.66 | 0.687689 |
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Target: 5'- uUUGGCccuggGCUGCGGCuuGGGccGgCCCAGCg -3' miRNA: 3'- cAGCUGa----CGAUGCUG--CCU--UgGGGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 41030 | 0.66 | 0.687689 |
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Target: 5'- -aCGGCUGCUGCuGGCcGAGCaccugaaacugCCAGCg -3' miRNA: 3'- caGCUGACGAUG-CUGcCUUGg----------GGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 20425 | 0.66 | 0.687689 |
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Target: 5'- -aCuGCUGCUggACGGCguuGGAGgcaucCCCCAGCg -3' miRNA: 3'- caGcUGACGA--UGCUG---CCUU-----GGGGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 2787 | 0.66 | 0.687689 |
|
Target: 5'- -gCGGCgGCgcuccagGCGGCGGcgaagcGCCUCGGCa -3' miRNA: 3'- caGCUGaCGa------UGCUGCCu-----UGGGGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 20590 | 0.66 | 0.665771 |
|
Target: 5'- -gCGACUGCUgcucggcaGCGccGCGGAAgUCgGGCg -3' miRNA: 3'- caGCUGACGA--------UGC--UGCCUUgGGgUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 5822 | 0.67 | 0.654759 |
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Target: 5'- --gGGCUGCUGgaGGCGGAuggccuCCCguGCc -3' miRNA: 3'- cagCUGACGAUg-CUGCCUu-----GGGguCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 16714 | 0.67 | 0.632682 |
|
Target: 5'- aGUCGAgcgagcacuCUGCUcuGCGAUGGcauCCUCGGUg -3' miRNA: 3'- -CAGCU---------GACGA--UGCUGCCuu-GGGGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 27342 | 0.73 | 0.295428 |
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Target: 5'- -cCGGCUGCUGCGGCGccGGGCUCUgcugGGCg -3' miRNA: 3'- caGCUGACGAUGCUGC--CUUGGGG----UCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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