Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11641 | 5' | -52 | NC_003094.2 | + | 28165 | 0.67 | 0.956198 |
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Target: 5'- -gUUGGCCACgGAcaaguuguAgGCGCGACgGGCa -3' miRNA: 3'- caAGUUGGUGgCU--------UgCGUGUUGgCCG- -5' |
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| 11641 | 5' | -52 | NC_003094.2 | + | 99905 | 0.67 | 0.958898 |
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Target: 5'- -cUCGGCUGCagguagcuuaGAACGCAUAauuaaagcguaaauACCGGCu -3' miRNA: 3'- caAGUUGGUGg---------CUUGCGUGU--------------UGGCCG- -5' |
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| 11641 | 5' | -52 | NC_003094.2 | + | 39281 | 0.67 | 0.963603 |
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Target: 5'- aUUCGGaauCUGGACGCGCAguuauAUCGGCa -3' miRNA: 3'- cAAGUUgguGGCUUGCGUGU-----UGGCCG- -5' |
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| 11641 | 5' | -52 | NC_003094.2 | + | 41172 | 0.67 | 0.966957 |
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Target: 5'- --aCAACCACauacAACGU--GACCGGCg -3' miRNA: 3'- caaGUUGGUGgc--UUGCGugUUGGCCG- -5' |
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| 11641 | 5' | -52 | NC_003094.2 | + | 45707 | 0.67 | 0.970086 |
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Target: 5'- --aCAAUCGCCaAGCGUGCcauuACCGGUu -3' miRNA: 3'- caaGUUGGUGGcUUGCGUGu---UGGCCG- -5' |
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| 11641 | 5' | -52 | NC_003094.2 | + | 81701 | 0.74 | 0.691661 |
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Target: 5'- -gUCGACCGcCCGAACGUcuACGGCCGccGCc -3' miRNA: 3'- caAGUUGGU-GGCUUGCG--UGUUGGC--CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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