Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11661 | 5' | -49.2 | NC_003102.1 | + | 36124 | 0.66 | 0.998388 |
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Target: 5'- gUUCAUCGGauccaaagCGGCUGCg---GCGg -3' miRNA: 3'- gAAGUAGCUgaa-----GCCGAUGacaaCGC- -5' |
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| 11661 | 5' | -49.2 | NC_003102.1 | + | 32399 | 0.66 | 0.997676 |
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Target: 5'- uUUCAgaCGGCUgCGGCUGCg---GCGg -3' miRNA: 3'- gAAGUa-GCUGAaGCCGAUGacaaCGC- -5' |
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| 11661 | 5' | -49.2 | NC_003102.1 | + | 73996 | 0.67 | 0.99469 |
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Target: 5'- --gCGUCGGCauaUCGGCgu-UGUUGCGc -3' miRNA: 3'- gaaGUAGCUGa--AGCCGaugACAACGC- -5' |
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| 11661 | 5' | -49.2 | NC_003102.1 | + | 4402 | 0.68 | 0.990523 |
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Target: 5'- gUUCAUCGGgUUCgacgGGCUGCUGaucaucgagcUGCGg -3' miRNA: 3'- gAAGUAGCUgAAG----CCGAUGACa---------ACGC- -5' |
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| 11661 | 5' | -49.2 | NC_003102.1 | + | 118019 | 0.69 | 0.977518 |
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Target: 5'- -aUCGUCGAucuCUUCGGCguagGCgGUUGUu -3' miRNA: 3'- gaAGUAGCU---GAAGCCGa---UGaCAACGc -5' |
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| 11661 | 5' | -49.2 | NC_003102.1 | + | 46349 | 0.71 | 0.95456 |
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Target: 5'- --cCGUCGACUUCGGaggacgUGCUGUcUGCc -3' miRNA: 3'- gaaGUAGCUGAAGCCg-----AUGACA-ACGc -5' |
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| 11661 | 5' | -49.2 | NC_003102.1 | + | 3734 | 0.76 | 0.783531 |
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Target: 5'- --aCAUCGGCggUGGCUGCUGcUGCu -3' miRNA: 3'- gaaGUAGCUGaaGCCGAUGACaACGc -5' |
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| 11661 | 5' | -49.2 | NC_003102.1 | + | 98384 | 0.78 | 0.680714 |
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Target: 5'- -aUCGUCGACgUCGGCUGuCUGUcUGCu -3' miRNA: 3'- gaAGUAGCUGaAGCCGAU-GACA-ACGc -5' |
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| 11661 | 5' | -49.2 | NC_003102.1 | + | 35022 | 1.11 | 0.009582 |
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Target: 5'- cCUUCAUCGACUUCGGCUACUGUUGCGa -3' miRNA: 3'- -GAAGUAGCUGAAGCCGAUGACAACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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