Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11665 | 5' | -45.2 | NC_003102.1 | + | 52994 | 0.66 | 0.999984 |
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Target: 5'- ---aUGGCGCGGUCcGCGAUaGUAu- -3' miRNA: 3'- uuuaACCGUGUUAGaUGCUAgCAUca -5' |
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| 11665 | 5' | -45.2 | NC_003102.1 | + | 96494 | 0.66 | 0.999958 |
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Target: 5'- ---cUGGCcgACAAUCUgAUGAUCGgcGUc -3' miRNA: 3'- uuuaACCG--UGUUAGA-UGCUAGCauCA- -5' |
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| 11665 | 5' | -45.2 | NC_003102.1 | + | 63090 | 0.68 | 0.999703 |
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Target: 5'- ----cGGCACGGUCcagauCGAUCGUGu- -3' miRNA: 3'- uuuaaCCGUGUUAGau---GCUAGCAUca -5' |
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| 11665 | 5' | -45.2 | NC_003102.1 | + | 50609 | 0.69 | 0.999243 |
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Target: 5'- ----cGGCGa---CUACGAUCGUAGa -3' miRNA: 3'- uuuaaCCGUguuaGAUGCUAGCAUCa -5' |
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| 11665 | 5' | -45.2 | NC_003102.1 | + | 97702 | 0.71 | 0.996385 |
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Target: 5'- ----cGGCACGGUCcACGAUC-UAGUu -3' miRNA: 3'- uuuaaCCGUGUUAGaUGCUAGcAUCA- -5' |
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| 11665 | 5' | -45.2 | NC_003102.1 | + | 91483 | 0.72 | 0.987716 |
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Target: 5'- ----gGGCAUAAUgUACGAUCGgUAGa -3' miRNA: 3'- uuuaaCCGUGUUAgAUGCUAGC-AUCa -5' |
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| 11665 | 5' | -45.2 | NC_003102.1 | + | 50778 | 1.05 | 0.043278 |
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Target: 5'- cAAAUUGGCACAAUCUACGAUCGUAGUc -3' miRNA: 3'- -UUUAACCGUGUUAGAUGCUAGCAUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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