Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11667 | 3' | -54.5 | NC_003102.1 | + | 81293 | 0.66 | 0.927091 |
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Target: 5'- --uGGCGAgGGGCagcucaGcuACGCCGAc- -3' miRNA: 3'- cuuUCGCUgCCCGg-----CuuUGCGGCUaa -5' |
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| 11667 | 3' | -54.5 | NC_003102.1 | + | 72605 | 0.67 | 0.882974 |
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Target: 5'- ---cGUGAUGGGCacagauCGAAACGCUGGg- -3' miRNA: 3'- cuuuCGCUGCCCG------GCUUUGCGGCUaa -5' |
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| 11667 | 3' | -54.5 | NC_003102.1 | + | 17752 | 0.67 | 0.87571 |
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Target: 5'- --cGGCGGCGGuGCCGG--UGUCGGUg -3' miRNA: 3'- cuuUCGCUGCC-CGGCUuuGCGGCUAa -5' |
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| 11667 | 3' | -54.5 | NC_003102.1 | + | 133948 | 0.71 | 0.702964 |
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Target: 5'- ---uGCGAC-GGCUGAGACGCCGc-- -3' miRNA: 3'- cuuuCGCUGcCCGGCUUUGCGGCuaa -5' |
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| 11667 | 3' | -54.5 | NC_003102.1 | + | 113816 | 0.71 | 0.692668 |
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Target: 5'- cGAAAcGUGGCGGGCgCG-GGCGUCGAUUu -3' miRNA: 3'- -CUUU-CGCUGCCCG-GCuUUGCGGCUAA- -5' |
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| 11667 | 3' | -54.5 | NC_003102.1 | + | 48998 | 0.71 | 0.692668 |
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Target: 5'- --cGGCGACGGcGgCGAAACGCCcGUUc -3' miRNA: 3'- cuuUCGCUGCC-CgGCUUUGCGGcUAA- -5' |
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| 11667 | 3' | -54.5 | NC_003102.1 | + | 129182 | 0.72 | 0.640581 |
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Target: 5'- cGAGuAGCGGCGGccggcGCCGAcggagcaguaGACGCCGAa- -3' miRNA: 3'- -CUU-UCGCUGCC-----CGGCU----------UUGCGGCUaa -5' |
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| 11667 | 3' | -54.5 | NC_003102.1 | + | 62192 | 1.06 | 0.005115 |
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Target: 5'- cGAAAGCGACGGGCCGAAACGCCGAUUg -3' miRNA: 3'- -CUUUCGCUGCCCGGCUUUGCGGCUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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