Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11671 | 3' | -50.2 | NC_003102.1 | + | 74228 | 1.1 | 0.008642 |
Target: 5'- cCGUCGUCGCCUCUAGCCAAAAAUCCAu -3' miRNA: 3'- -GCAGCAGCGGAGAUCGGUUUUUAGGU- -5' |
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11671 | 3' | -50.2 | NC_003102.1 | + | 49275 | 0.71 | 0.909012 |
Target: 5'- uCG-CGUCGCgUCUGGCCAcccAUCUAc -3' miRNA: 3'- -GCaGCAGCGgAGAUCGGUuuuUAGGU- -5' |
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11671 | 3' | -50.2 | NC_003102.1 | + | 108546 | 0.7 | 0.932336 |
Target: 5'- uCGUCGUCGUCgUCggAGCCGGAA--CCGa -3' miRNA: 3'- -GCAGCAGCGG-AGa-UCGGUUUUuaGGU- -5' |
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11671 | 3' | -50.2 | NC_003102.1 | + | 41043 | 0.7 | 0.942429 |
Target: 5'- gGUUGUCGUCUCUAuacccgcugacGCCG---GUCCAa -3' miRNA: 3'- gCAGCAGCGGAGAU-----------CGGUuuuUAGGU- -5' |
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11671 | 3' | -50.2 | NC_003102.1 | + | 81154 | 0.68 | 0.972779 |
Target: 5'- aGUCGUCGCCg--AGCCuc-AAUCa- -3' miRNA: 3'- gCAGCAGCGGagaUCGGuuuUUAGgu -5' |
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11671 | 3' | -50.2 | NC_003102.1 | + | 101410 | 0.68 | 0.97807 |
Target: 5'- uGUUGUCGUUUCUaaAGUCAuugcguGAUCCAu -3' miRNA: 3'- gCAGCAGCGGAGA--UCGGUuu----UUAGGU- -5' |
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11671 | 3' | -50.2 | NC_003102.1 | + | 61876 | 0.68 | 0.980411 |
Target: 5'- gCGUCGUCGCCg-UAGUgauCAAugucuGAUCCGu -3' miRNA: 3'- -GCAGCAGCGGagAUCG---GUUu----UUAGGU- -5' |
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11671 | 3' | -50.2 | NC_003102.1 | + | 114488 | 0.67 | 0.990756 |
Target: 5'- gCGUCGaUCGUCUUUGaucGCgCAAAAAUCUg -3' miRNA: 3'- -GCAGC-AGCGGAGAU---CG-GUUUUUAGGu -5' |
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11671 | 3' | -50.2 | NC_003102.1 | + | 40158 | 0.66 | 0.994817 |
Target: 5'- -cUCGUCGCCccaUCUcGCCGAAgcGAUCg- -3' miRNA: 3'- gcAGCAGCGG---AGAuCGGUUU--UUAGgu -5' |
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11671 | 3' | -50.2 | NC_003102.1 | + | 109868 | 0.66 | 0.994817 |
Target: 5'- uCGUCGUCGCCgUCU--UCAAAAuuuucaaacgcAUCCGu -3' miRNA: 3'- -GCAGCAGCGG-AGAucGGUUUU-----------UAGGU- -5' |
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11671 | 3' | -50.2 | NC_003102.1 | + | 72034 | 0.66 | 0.995564 |
Target: 5'- aCGUCGcauuccuugCGCCUCUGuCCAc--GUCCAu -3' miRNA: 3'- -GCAGCa--------GCGGAGAUcGGUuuuUAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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