Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11675 | 5' | -56.5 | NC_003102.1 | + | 4408 | 0.66 | 0.823379 |
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Target: 5'- cGGGUUCGACGgGCuGCUgaucaucgaGCUGCGGu -3' miRNA: 3'- uCUCAAGCUGCgCG-CGGua-------CGACGUU- -5' |
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| 11675 | 5' | -56.5 | NC_003102.1 | + | 14161 | 0.67 | 0.777794 |
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Target: 5'- cGAGUcUCGugGCG-GCgGUuGCUGCAc -3' miRNA: 3'- uCUCA-AGCugCGCgCGgUA-CGACGUu -5' |
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| 11675 | 5' | -56.5 | NC_003102.1 | + | 121106 | 0.67 | 0.777794 |
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Target: 5'- uGAugUCGGCGaCGaCGCCGUGCgGCAu -3' miRNA: 3'- uCUcaAGCUGC-GC-GCGGUACGaCGUu -5' |
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| 11675 | 5' | -56.5 | NC_003102.1 | + | 115338 | 0.68 | 0.748737 |
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Target: 5'- cGAGUUacACGaGCGCCGUGUUGCu- -3' miRNA: 3'- uCUCAAgcUGCgCGCGGUACGACGuu -5' |
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| 11675 | 5' | -56.5 | NC_003102.1 | + | 4165 | 0.7 | 0.615071 |
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Target: 5'- aAGAGUUgGACGUGCGCgaCAUaaaGUUGCAu -3' miRNA: 3'- -UCUCAAgCUGCGCGCG--GUA---CGACGUu -5' |
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| 11675 | 5' | -56.5 | NC_003102.1 | + | 133856 | 0.7 | 0.604638 |
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Target: 5'- uGGAGUgauggacagCGACGaCGCgGCCGaGCUGCAu -3' miRNA: 3'- -UCUCAa--------GCUGC-GCG-CGGUaCGACGUu -5' |
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| 11675 | 5' | -56.5 | NC_003102.1 | + | 99510 | 1.06 | 0.003155 |
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Target: 5'- aAGAGUUCGACGCGCGCCAUGCUGCAAc -3' miRNA: 3'- -UCUCAAGCUGCGCGCGGUACGACGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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