Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 11678 | 5' | -58.6 | NC_003102.1 | + | 106109 | 1.09 | 0.001954 |
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Target: 5'- aUCGACGUCGAGGCGUCCUCCGUCGCAu -3' miRNA: 3'- -AGCUGCAGCUCCGCAGGAGGCAGCGU- -5' |
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| 11678 | 5' | -58.6 | NC_003102.1 | + | 120532 | 0.75 | 0.313212 |
|
Target: 5'- aUCGACGUCGugcuuuAGaCGUCCUCCGUacaGCAu -3' miRNA: 3'- -AGCUGCAGC------UCcGCAGGAGGCAg--CGU- -5' |
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| 11678 | 5' | -58.6 | NC_003102.1 | + | 133770 | 0.71 | 0.532427 |
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Target: 5'- gUCGAgCGUCuGAgagacgagcGGCGUCUcagCCGUCGCAa -3' miRNA: 3'- -AGCU-GCAG-CU---------CCGCAGGa--GGCAGCGU- -5' |
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| 11678 | 5' | -58.6 | NC_003102.1 | + | 63575 | 0.71 | 0.542091 |
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Target: 5'- aUCGACG-CGGGaGCGgcUCCUCCGuaaUCGUAu -3' miRNA: 3'- -AGCUGCaGCUC-CGC--AGGAGGC---AGCGU- -5' |
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| 11678 | 5' | -58.6 | NC_003102.1 | + | 93416 | 0.69 | 0.640939 |
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Target: 5'- gUCGACgGUCGAGGCGagagcgCCG-CGCAa -3' miRNA: 3'- -AGCUG-CAGCUCCGCagga--GGCaGCGU- -5' |
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| 11678 | 5' | -58.6 | NC_003102.1 | + | 130886 | 0.69 | 0.640939 |
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Target: 5'- gUUGGCGUUGGcGCGUCCUaugcaaGUCGCGu -3' miRNA: 3'- -AGCUGCAGCUcCGCAGGAgg----CAGCGU- -5' |
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| 11678 | 5' | -58.6 | NC_003102.1 | + | 110363 | 0.69 | 0.650902 |
|
Target: 5'- aCGAucaUGUCGAcGGCGugUCCgCUGUCGCAg -3' miRNA: 3'- aGCU---GCAGCU-CCGC--AGGaGGCAGCGU- -5' |
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| 11678 | 5' | -58.6 | NC_003102.1 | + | 73323 | 0.67 | 0.767049 |
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Target: 5'- gUCGuCGUCGucGuCGUCCUCguCGUCGUc -3' miRNA: 3'- -AGCuGCAGCucC-GCAGGAG--GCAGCGu -5' |
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| 11678 | 5' | -58.6 | NC_003102.1 | + | 58651 | 0.67 | 0.767049 |
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Target: 5'- gUCGuCGUCGA--CGUCg-CCGUCGCAg -3' miRNA: 3'- -AGCuGCAGCUccGCAGgaGGCAGCGU- -5' |
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| 11678 | 5' | -58.6 | NC_003102.1 | + | 81556 | 0.67 | 0.77619 |
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Target: 5'- gUCGAaGUCGAcGCGgUCgCCGUCGCAg -3' miRNA: 3'- -AGCUgCAGCUcCGCaGGaGGCAGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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