miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
11681 5' -54.6 NC_003102.1 + 134133 0.71 0.707604
Target:  5'- -gAUGCacCGCaGUCGCUUGUCGAUguugUCCGc -3'
miRNA:   3'- caUACG--GCG-CAGCGGACAGCUA----AGGU- -5'
11681 5' -54.6 NC_003102.1 + 134025 0.69 0.81342
Target:  5'- cUcgGCCGCGUCGUcgCUGUCcAUcacUCCAa -3'
miRNA:   3'- cAuaCGGCGCAGCG--GACAGcUA---AGGU- -5'
11681 5' -54.6 NC_003102.1 + 133752 0.66 0.933905
Target:  5'- ---cGCCGUaguGUCGCCgcaGUCGAgcgUCUg -3'
miRNA:   3'- cauaCGGCG---CAGCGGa--CAGCUa--AGGu -5'
11681 5' -54.6 NC_003102.1 + 132009 1.08 0.003918
Target:  5'- uGUAUGCCGCGUCGCCUGUCGAUUCCAu -3'
miRNA:   3'- -CAUACGGCGCAGCGGACAGCUAAGGU- -5'
11681 5' -54.6 NC_003102.1 + 91337 0.66 0.938865
Target:  5'- ---gGCCGCGUC-CgaGUCGAaUCUAc -3'
miRNA:   3'- cauaCGGCGCAGcGgaCAGCUaAGGU- -5'
11681 5' -54.6 NC_003102.1 + 87604 0.68 0.847439
Target:  5'- -gGUGCCaccgcCGUCGCCgacGUCGAUcgaucuUCCAu -3'
miRNA:   3'- caUACGGc----GCAGCGGa--CAGCUA------AGGU- -5'
11681 5' -54.6 NC_003102.1 + 48862 0.67 0.885375
Target:  5'- ---cGCCGCGUCGCUUGaaugCGA--CCu -3'
miRNA:   3'- cauaCGGCGCAGCGGACa---GCUaaGGu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.