Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11683 | 5' | -52.9 | NC_003102.1 | + | 33097 | 0.69 | 0.884138 |
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Target: 5'- -cUGUCGgcGGCGGcGGCGAcuGCACGGCGu -3' miRNA: 3'- gaGCAGC--UCGUCuCUGCU--UGUGUCGU- -5' |
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| 11683 | 5' | -52.9 | NC_003102.1 | + | 59518 | 0.68 | 0.922578 |
|
Target: 5'- gUCuUCGAGCGGcGACGcGACgGCGGCAa -3' miRNA: 3'- gAGcAGCUCGUCuCUGC-UUG-UGUCGU- -5' |
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| 11683 | 5' | -52.9 | NC_003102.1 | + | 93413 | 0.67 | 0.956065 |
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Target: 5'- uUCGUCGAcgGUcGAGGCGAgaGCGCcGCGc -3' miRNA: 3'- gAGCAGCU--CGuCUCUGCU--UGUGuCGU- -5' |
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| 11683 | 5' | -52.9 | NC_003102.1 | + | 102040 | 0.67 | 0.952022 |
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Target: 5'- aUCGaagCGgcGGCGGuGGCGGugGCGGCGg -3' miRNA: 3'- gAGCa--GC--UCGUCuCUGCUugUGUCGU- -5' |
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| 11683 | 5' | -52.9 | NC_003102.1 | + | 112998 | 0.7 | 0.853765 |
|
Target: 5'- gUCGUCGuGGCGGugguggugucGGCGGugGCGGCAg -3' miRNA: 3'- gAGCAGC-UCGUCu---------CUGCUugUGUCGU- -5' |
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| 11683 | 5' | -52.9 | NC_003102.1 | + | 123377 | 0.68 | 0.933394 |
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Target: 5'- -gCGUCGAGUGGcGACGAgGCGgAGUAg -3' miRNA: 3'- gaGCAGCUCGUCuCUGCU-UGUgUCGU- -5' |
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| 11683 | 5' | -52.9 | NC_003102.1 | + | 126286 | 0.67 | 0.947736 |
|
Target: 5'- -cCGUuucCGAGCAGcgGGACGGucaGCAGCGg -3' miRNA: 3'- gaGCA---GCUCGUC--UCUGCUug-UGUCGU- -5' |
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| 11683 | 5' | -52.9 | NC_003102.1 | + | 133769 | 0.7 | 0.837284 |
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Target: 5'- --aGUCGAGCgucugAGAGACGA--GCGGCGu -3' miRNA: 3'- gagCAGCUCG-----UCUCUGCUugUGUCGU- -5' |
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| 11683 | 5' | -52.9 | NC_003102.1 | + | 134898 | 1.09 | 0.005173 |
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Target: 5'- cCUCGUCGAGCAGAGACGAACACAGCAu -3' miRNA: 3'- -GAGCAGCUCGUCUCUGCUUGUGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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