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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11684 | 3' | -46.6 | NC_003102.1 | + | 32134 | 0.71 | 0.992612 |
|
Target: 5'- --cGUCGcaGUCUUCCAgaUCGUUGUu -3' miRNA: 3'- guuCAGCa-CAGAAGGUaaAGCAACGc -5' |
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| 11684 | 3' | -46.6 | NC_003102.1 | + | 13104 | 0.69 | 0.998027 |
|
Target: 5'- --cGUCGUGUCcacUUCCuUUUCGagcgUGCGc -3' miRNA: 3'- guuCAGCACAG---AAGGuAAAGCa---ACGC- -5' |
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| 11684 | 3' | -46.6 | NC_003102.1 | + | 8455 | 0.7 | 0.99533 |
|
Target: 5'- gAGGUCGcGUCUUCCGUUUCuucuaaacGCGc -3' miRNA: 3'- gUUCAGCaCAGAAGGUAAAGcaa-----CGC- -5' |
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| 11684 | 3' | -46.6 | NC_003102.1 | + | 3843 | 0.73 | 0.973845 |
|
Target: 5'- --cGUCGUGUCUUUCAUUUCugagGCu -3' miRNA: 3'- guuCAGCACAGAAGGUAAAGcaa-CGc -5' |
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| 11684 | 3' | -46.6 | NC_003102.1 | + | 3796 | 0.66 | 0.999861 |
|
Target: 5'- cUAAGUcuacugcCGUGUCUUCCAUUUCuaaGUcuacUGCc -3' miRNA: 3'- -GUUCA-------GCACAGAAGGUAAAG---CA----ACGc -5' |
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| 11684 | 3' | -46.6 | NC_003102.1 | + | 3642 | 0.7 | 0.996029 |
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Target: 5'- -cGGUCGUGUCUUCCAcUUCcUU-CGa -3' miRNA: 3'- guUCAGCACAGAAGGUaAAGcAAcGC- -5' |
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| 11684 | 3' | -46.6 | NC_003102.1 | + | 3443 | 1.09 | 0.02212 |
|
Target: 5'- uCAAGUCGUGUCUUCCAUUUCGUUGCGu -3' miRNA: 3'- -GUUCAGCACAGAAGGUAAAGCAACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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