Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11686 | 3' | -54.7 | NC_003102.1 | + | 23460 | 0.66 | 0.928698 |
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Target: 5'- gCGCCcggucucuuAUUGCCGCCcugAUCGAcgUaacgGGCCGu -3' miRNA: 3'- -GCGG---------UAACGGUGG---UAGCUuaG----CCGGU- -5' |
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| 11686 | 3' | -54.7 | NC_003102.1 | + | 21472 | 0.7 | 0.776679 |
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Target: 5'- aGCCGgcaUGUUACCAUCucGAUCGGUCGg -3' miRNA: 3'- gCGGUa--ACGGUGGUAGc-UUAGCCGGU- -5' |
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| 11686 | 3' | -54.7 | NC_003102.1 | + | 21040 | 0.73 | 0.604052 |
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Target: 5'- uGCCAccGCCGCCccaaGUCGAcUCGGCUc -3' miRNA: 3'- gCGGUaaCGGUGG----UAGCUuAGCCGGu -5' |
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| 11686 | 3' | -54.7 | NC_003102.1 | + | 19546 | 0.68 | 0.844994 |
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Target: 5'- cCGCCGaucucGCUcgucucuccuaaucACCAUCGAGUCGGgCAc -3' miRNA: 3'- -GCGGUaa---CGG--------------UGGUAGCUUAGCCgGU- -5' |
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| 11686 | 3' | -54.7 | NC_003102.1 | + | 15726 | 1.08 | 0.003703 |
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Target: 5'- uCGCCAUUGCCACCAUCGAAUCGGCCAc -3' miRNA: 3'- -GCGGUAACGGUGGUAGCUUAGCCGGU- -5' |
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| 11686 | 3' | -54.7 | NC_003102.1 | + | 6713 | 0.66 | 0.928698 |
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Target: 5'- uGCCGcUGCCGCCgguGUUGGcgCuGCCGa -3' miRNA: 3'- gCGGUaACGGUGG---UAGCUuaGcCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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