Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11686 | 3' | -54.7 | NC_003102.1 | + | 119479 | 0.71 | 0.707604 |
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Target: 5'- aCGCCAccggcGCCGCCGUUug--CGGCCAa -3' miRNA: 3'- -GCGGUaa---CGGUGGUAGcuuaGCCGGU- -5' |
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| 11686 | 3' | -54.7 | NC_003102.1 | + | 44238 | 0.71 | 0.676813 |
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Target: 5'- aCGCUAUUGUgaugaCAUCAUCGAuccAUCGGUCGc -3' miRNA: 3'- -GCGGUAACG-----GUGGUAGCU---UAGCCGGU- -5' |
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| 11686 | 3' | -54.7 | NC_003102.1 | + | 59347 | 0.72 | 0.656081 |
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Target: 5'- -aCCGUUGCCGCCGUCGccGUC-GCCGc -3' miRNA: 3'- gcGGUAACGGUGGUAGCu-UAGcCGGU- -5' |
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| 11686 | 3' | -54.7 | NC_003102.1 | + | 21040 | 0.73 | 0.604052 |
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Target: 5'- uGCCAccGCCGCCccaaGUCGAcUCGGCUc -3' miRNA: 3'- gCGGUaaCGGUGG----UAGCUuAGCCGGu -5' |
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| 11686 | 3' | -54.7 | NC_003102.1 | + | 71483 | 0.74 | 0.536327 |
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Target: 5'- cCGCCGUUGCCGCCucuGUCGcuAUCggaauuuacauuuauGGCCAg -3' miRNA: 3'- -GCGGUAACGGUGG---UAGCu-UAG---------------CCGGU- -5' |
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| 11686 | 3' | -54.7 | NC_003102.1 | + | 15726 | 1.08 | 0.003703 |
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Target: 5'- uCGCCAUUGCCACCAUCGAAUCGGCCAc -3' miRNA: 3'- -GCGGUAACGGUGGUAGCUUAGCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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