Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11686 | 3' | -54.7 | NC_003102.1 | + | 39304 | 0.69 | 0.791666 |
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Target: 5'- aGCCGaaaugucuaacggUGCgCGCCGUcucgcgcaaacCGAAUCGGCCAu -3' miRNA: 3'- gCGGUa------------ACG-GUGGUA-----------GCUUAGCCGGU- -5' |
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| 11686 | 3' | -54.7 | NC_003102.1 | + | 57921 | 0.69 | 0.81342 |
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Target: 5'- uCGCguUcGCCGCCGUCGAAgguauauucuuuUCGGgCAg -3' miRNA: 3'- -GCGguAaCGGUGGUAGCUU------------AGCCgGU- -5' |
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| 11686 | 3' | -54.7 | NC_003102.1 | + | 82482 | 0.68 | 0.855458 |
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Target: 5'- aCGCUGUUGCucUugC-UCGAAUCGGCgGa -3' miRNA: 3'- -GCGGUAACG--GugGuAGCUUAGCCGgU- -5' |
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| 11686 | 3' | -54.7 | NC_003102.1 | + | 69660 | 0.68 | 0.863267 |
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Target: 5'- aCGCCAccuUUGUCACCAUCGGAccguauUCGaUCAc -3' miRNA: 3'- -GCGGU---AACGGUGGUAGCUU------AGCcGGU- -5' |
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| 11686 | 3' | -54.7 | NC_003102.1 | + | 26538 | 0.67 | 0.892288 |
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Target: 5'- gCGUCAUaUG-CGCCAUCuaaaaAGUCGGCCAc -3' miRNA: 3'- -GCGGUA-ACgGUGGUAGc----UUAGCCGGU- -5' |
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| 11686 | 3' | -54.7 | NC_003102.1 | + | 15726 | 1.08 | 0.003703 |
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Target: 5'- uCGCCAUUGCCACCAUCGAAUCGGCCAc -3' miRNA: 3'- -GCGGUAACGGUGGUAGCUUAGCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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