miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
11686 5' -48.8 NC_003102.1 + 15763 1.09 0.011583
Target:  5'- cACUAAGACGCCGAUGAUAGCAUCAGAc -3'
miRNA:   3'- -UGAUUCUGCGGCUACUAUCGUAGUCU- -5'
11686 5' -48.8 NC_003102.1 + 75730 0.74 0.867257
Target:  5'- --cGAGACGgacaucgaaaCCGAUGAUGGUGUCGGGa -3'
miRNA:   3'- ugaUUCUGC----------GGCUACUAUCGUAGUCU- -5'
11686 5' -48.8 NC_003102.1 + 20885 0.69 0.98362
Target:  5'- cUUGGGGCGgCGGUGGcAGCAuuUCGGAc -3'
miRNA:   3'- uGAUUCUGCgGCUACUaUCGU--AGUCU- -5'
11686 5' -48.8 NC_003102.1 + 82040 0.66 0.998021
Target:  5'- -gUGGGACuGUCGAUGGU-GCGUCAu- -3'
miRNA:   3'- ugAUUCUG-CGGCUACUAuCGUAGUcu -5'
11686 5' -48.8 NC_003102.1 + 74777 0.67 0.994546
Target:  5'- cACUucGGAUGCCGAauuUGAaauGCAUCGGu -3'
miRNA:   3'- -UGAu-UCUGCGGCU---ACUau-CGUAGUCu -5'
11686 5' -48.8 NC_003102.1 + 4955 0.68 0.98837
Target:  5'- uACUAccgauGACGCUGAUGAUucgGGCGuggcggccaccacaUCAGAc -3'
miRNA:   3'- -UGAUu----CUGCGGCUACUA---UCGU--------------AGUCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.